Toxicity of parasporin-4 and health effects of pro-parasporin-4 diet in mice.
Toxicity of parasporin-4 and health effects of pro-parasporin-4 diet in mice.
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DOI：
10.3390/toxins6072115
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发表时间：
2014-07-16
期刊：
影响因子：
4.2
通讯作者：
Mizuki E
中科院分区：
文献类型：
--
作者：
Okumura S;Koga H;Inouye K;Mizuki E
副孢子蛋白-4（Parasporin-4，PS4）是苏云金芽孢杆菌A1470菌株产生的一种气溶素型β-孔形成毒素。它对人类癌细胞系表现出特异性细胞毒性;因此，预计可用于诊断和治疗特定类型的癌细胞。我们研究了PS4对ICR小鼠的急性毒性。经皮下给药，LD 50值为160 μg/kg。钾，铵，镁离子，肌酐，尿素氮减少尿中注射PS4。同时，小鼠血清肌酐和尿素氮升高。这些结果表明，PS4损害小鼠的肾功能。PS4是从Pro-parasporin-4（ProPS 4）中通过加工获得的，ProPS 4是由重组大肠杆菌作为包涵体产生的。ProPS 4包涵体在弱酸性溶液中溶解，并被胃蛋白酶激活，提示口服后在哺乳动物胃中溶解并激活。因此，检查了C57 BL/6 J小鼠口服给药的影响。虽然ProPS 4在小鼠消化道中被激活为PS4，但没有观察到任何严重的健康危害，并且体重变化没有显著差异。
Parasporin-4 (PS4) is an aerolysin-type β-pore-forming toxin produced by Bacillus thuringiensis strain A1470. It exhibits specific cytotoxicity against human cancer cell lines; therefore, it is expected to be useful for the diagnosis and treatment of particular types of cancer cells. We examined the acute toxicity of PS4 on ICR mice. The LD50 value was 160 μg/kg by a subcutaneous route. Potassium, ammonium, magnesium ion, creatinine, and urea nitrogen decreased in urine by the injection of PS4. Simultaneously, creatinine and urea nitrogen in mice serum increased. These results imply that PS4 impairs kidney function in mice. PS4 is obtained from Pro-parasporin-4 (ProPS4) by processing, and ProPS4 is produced by recombinant Escherichia coli as the inclusion body. The inclusion body of ProPS4 can be solubilized in a weak acid solution and activated by pepsin, implying that it would be solubilized and activated in the stomach of mammals after oral administration. Thus, the influence of the oral administration of it by C57BL/6J mice was examined. Although ProPS4 was activated to PS4 in the mouse digestive tract, any serious health hazard was not observed and there was no significant difference in body weight change.
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DOI：
10.1128/cdli.7.4.625-634.2000
发表时间：
2000-07-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Mizuki, E;Park, YS;Ohba, M
通讯作者：
Ohba, M
DOI：
10.1016/s1387-2656(08)00009-4
发表时间：
2008-01-01
期刊：
BIOTECHNOLOGY ANNUAL REVIEW, VOL 14
影响因子：
--
作者：
Okumura, Shiro;Saitoh, Hiroyuki;Inouye, Kuniyo
通讯作者：
Inouye, Kuniyo
影响因子：
2.7
作者：
Oneda, H;Inouye, K
通讯作者：
Inouye, K
DOI：
10.1099/00221287-92-1-11
发表时间：
1976-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
FACKRELL, HB;WISEMAN, GM
通讯作者：
WISEMAN, GM
影响因子：
2.7
作者：
Okumura, Shiro;Ishikawa, Tomoyuki;Mizuki, Eiichi
通讯作者：
Mizuki, Eiichi