Canine piroplasmids: Molecular detection and laboratory characterization in dogs from Brasilia, Brazil, with the first molecular evidence of dog exposure to a novel opossum-associated Babesia sp.
Canine piroplasmids: Molecular detection and laboratory characterization in dogs from Brasilia, Brazil, with the first molecular evidence of dog exposure to a novel opossum-associated Babesia sp.
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DOI：
10.1016/j.ttbdis.2023.102181
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发表时间：
2023-07
影响因子：
3.2
通讯作者：
Paludo GR
中科院分区：
文献类型：
--
作者：
de Oliveira CM;Yang TS;Duarte MA;Marr H;McManus CM;André MR;Birkenheuer AJ;Paludo GR
犬的梨质粒感染可由巴贝斯虫、希勒氏菌和兰格氏菌引起。在巴西，由沃氏巴贝斯虫引起的犬巴贝斯虫病是一种地方性疾病，据报道在全国范围内流行。另一方面，迄今为止仅在南部和东南部地区描述了由小孢子虫引起的rangelelosis。尽管如此，分析这些血原虫的实验室和分子特征的研究仍然很少。为了调查巴西中西部地区螺质粒的发生、实验室特征、分子特征和多样性，对来自巴西中西部联邦区Brasília的276只家犬的血液样本进行了调查。采用针对线粒体大亚基核糖体DNA （LSU4）的宽范围定量PCR （qPCR）检测梨质粒DNA。其中，9.7%（27/276）为巴贝斯虫，与美国巴贝斯虫分离株的同源性为98-100%。基于部分18S rRNA序列两两比对（- 250 bp）， 1.4%（4/276）的序列与B. vogeli的同源性仅为76.8%，与负鼠相关巴贝斯虫sp. （MW290046-53）的同源性为100%。这些发现表明，来自巴西的狗接触了最近从白耳负鼠中分离出来的巴贝斯虫。所有被分析的狗都没有阳性的氏杆菌或R. vitalii。随后，将所有阳性序列提交给基于18S rRNA、cox-1和cytb基因的另外三次PCR分析，旨在进行单倍型网络分析。利用cox-1序列进行单倍型网络分析，结果显示存在6种不同的单倍型；其中一个与来自巴西、美国和印度的分离株共享。当包括同时感染其他媒介传播疾病的动物时，螺质粒阳性犬发生血小板减少症的几率比阴性犬高2.3倍。分子结果表明，比较的沃格里巴贝斯虫序列显示出低变异性，并且在巴西中西部的狗中暴露于假定的新型负鼠相关巴贝斯虫。
Canine piroplasmid infections can be caused by Babesia spp., Theileria spp. and Rangelia vitalii. In Brazil, canine babesiosis caused by Babesia vogeli is endemic and reported throughout the country. On the other hand, Rangeliosis caused by R. vitalii has only been described so far in the South and Southeast regions. Despite that, studies analyzing the laboratory and molecular characterization of these hemoprotozoa are still scarce. To investigate the occurrence, the laboratory features, the molecular characterization, and the diversity of piroplasmids from Midwestern Brazil, a survey was performed using blood samples obtained from 276 domestic dogs from Brasília, Federal District, Midwestern Brazil. A broad-range quantitative PCR (qPCR) targeting the mitochondrial large subunit ribosomal DNA (LSU4) was used to detect piroplasmid DNA. The overall molecular occurrence of piroplasmids was 11.2% (31/276), with 9.7% (27/276) of the sequences identified as Babesia vogeli (98–100% identity to B. vogeli isolate from the USA). Based on a partial 18S rRNA sequence pairwise alignment (−250 bp), 1.4% (4/276) of the sequences showed only 76.8% identity with B. vogeli but 100% identity with opossum-associated Babesia sp. (MW290046–53). These findings suggest the exposure of dogs from Brazil to a recently described Babesia sp. isolated from white-eared opossum. None of the analyzed dogs was positive for Theileria spp. or R. vitalii. Subsequently, all positive sequences were submitted to three additional PCR assays based on the 18S rRNA, cox-1, and cytb genes, aiming at performing a haplotype network analysis. Haplotype network using cox-1 sequences showed the presence of six different haplotypes of B. vogeli; one of them was shared with isolates from Brazil, the USA, and India. When including animals co-infected with other vector-borne diseases, piroplasmid-positive dogs had 2.3 times higher chance of having thrombocytopenia than the negative ones. The molecular results demonstrated that the compared Babesia vogeli sequences showed a low variability as well as evidence of exposure to a putative novel opossum-associated Babesia sp. in dogs from Midwestern Brazil.
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将Theileria annae重新分类为Babesia vulpes sp。十一月。
影响因子：
3.2
作者：
Baneth G;Florin-Christensen M;Cardoso L;Schnittger L
通讯作者：
Schnittger L
影响因子：
9.4
作者：
Birkenheuer, AJ;Levy, MG;Breitschwerdt, EB
通讯作者：
Breitschwerdt, EB
影响因子：
3.2
作者：
Diaz-Reganon, David;Agulla, Beatriz;Sainz, Angel
通讯作者：
Sainz, Angel
影响因子：
3.2
作者：
Baneth, Gad;Nachum-Biala, Yaarit;Aroch, Itamar
通讯作者：
Aroch, Itamar
影响因子：
3.2
作者：
Alvarado-Rybak M;Solano-Gallego L;Millán J
通讯作者：
Millán J