Immortalized CNS pericytes are quiescent smooth muscle actin-negative and pluripotent.
Immortalized CNS pericytes are quiescent smooth muscle actin-negative and pluripotent.
复制标题
DOI：
10.1016/j.mvr.2011.04.003
复制
发表时间：
2011-07
影响因子：
3.1
通讯作者：
Gow A
中科院分区：
文献类型：
--
作者：
Dore-Duffy P;Mehedi A;Wang X;Bradley M;Trotter R;Gow A
尽管100多年前由法国科学家Charles-Marie Benjamin Rouget鉴定，但微血管周细胞已被证明难以进行功能表征，部分原因是它们的数量相对较少且缺乏特异性细胞标记物。然而，最近的进展开始阐明这些细胞的多种生物学功能。周细胞被认为参与调节血管稳态和止血，以及作为成体干细胞的局部来源。为了进一步确定这些有趣的细胞的特性，我们从携带SV 40病毒靶T基因的温度敏感突变体的转基因小鼠（Immortomouse®）中分离了周细胞。该Immortopericyte（IMP）条件细胞系在长时间内是稳定的，并且在33°C在干扰素γ存在下不分化。在这些条件下，IMP是α肌肉肌动蛋白阴性的，并表现出多能表型，但在37°C下可被诱导沿着间充质和神经元谱系分化。或者，野生型周细胞和IMP的分化可以直接从培养物中的毛细血管诱导。最后，向纯化的IMP培养物中加入内皮细胞可能以细胞与细胞接触依赖的方式增强其自我更新和分化的速率。
Despite their identification more than 100 years ago by the French scientist Charles-Marie Benjamin Rouget, microvascular pericytes have proven difficult to functionally characterize, due in part to their relatively low numbers and the lack of specific cell markers. However, recent progress is beginning to shed light on the diverse biological functions of these cells. Pericytes are thought to be involved in regulating vascular homeostasis and hemostasis as well as serving as a local source of adult stem cells. To further define the properties of these intriguing cells, we have isolated pericytes from transgenic mice (Immortomouse®) harboring a temperature-sensitive mutant of the SV40 virus target T-gene. This Immortopericyte (IMP) conditional cell line is stable for long periods of time and, at 33°C in the presence of interferon gamma, does not differentiate. Under these conditions IMPs are alpha muscle actin-negative and exhibit a pluripotent phenotype, but can be induced to differentiate along both mesenchymal and neuronal lineages at 37°C. Alternatively, differentiation of wild type pericytes and IMPs can be induced directly from capillaries in culture. Finally, the addition of endothelial cells to purified IMP cultures augments their rate of self-renewal and differentiation, possibly in a cell-to-cell contact dependent manner.
登录
查看更多内容
骨髓来源的多能细胞是有助于血管化的周细胞
影响因子：
4.8
作者：
Cai, Xiaoxiao;Lin, Yunfeng;Grottkau, Brian E.
通讯作者：
Grottkau, Brian E.
DOI：
10.1083/jcb.200409107
发表时间：
2004-12-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Davidoff MS;Middendorff R;Enikolopov G;Riethmacher D;Holstein AF;Müller D
通讯作者：
Müller D
影响因子：
5.3
作者：
Arai, Ken;Lo, Eng H.
通讯作者：
Lo, Eng H.
影响因子：
20.3
作者：
Beltrami, Antonio R.;Cesselli, Daniela;Beltrami, Carlo A.
通讯作者：
Beltrami, Carlo A.
DOI：
10.1111/j.1749-6632.2009.04967.x
发表时间：
2009-01-01
期刊：
HEMATOPOIETIC STEM CELLS VII
影响因子：
--
作者：
Crisan, Mihaela;Chen, Chien-Wen;Peault, Bruno
通讯作者：
Peault, Bruno