Lactobacillus rhamnosus lowers zebrafish lipid content by changing gut microbiota and host transcription of genes involved in lipid metabolism.
Lactobacillus rhamnosus lowers zebrafish lipid content by changing gut microbiota and host transcription of genes involved in lipid metabolism.
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DOI：
10.1038/srep09336
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发表时间：
2015-03-30
影响因子：
4.6
通讯作者：
Carnevali O
中科院分区：
文献类型：
--
作者：
Falcinelli S;Picchietti S;Rodiles A;Cossignani L;Merrifield DL;Taddei AR;Maradonna F;Olivotto I;Gioacchini G;Carnevali O
微生物组在脂质代谢中起重要作用，但益生菌群落的引入如何影响宿主脂质代谢尚不清楚。采用多学科方法，我们利用斑马鱼模型，将高通量测序与生化、分子和形态学分析相结合，以评估肠道的变化，从而解决了这一知识差距。细菌16S文库分析表明，鼠李糖乳杆菌能够调节斑马鱼幼虫的肠道微生物群，提高厚壁菌门序列的丰度，降低放线菌门的丰度。肠道微生物组的变化通过诱导胆固醇和甘油三酯代谢相关基因（fit2、agpat4、dgat2、mgll、hnf4α、scap和cck）的转录下调来调节宿主脂质加工，同时降低总胆固醇和甘油三酯含量，增加脂肪酸水平。鼠李糖也增加了肠上皮微绒毛和肠细胞的长度，减少了肠上皮的脂滴大小。这些变化导致斑马鱼幼体生长加快。这项综合系统研究证明了益生菌对肠道微生物组的调节作用，突出了参与脂质代谢的新基因网络，为微生物组如何调节参与脂质代谢的分子提供了新的见解，并揭示了鼠李糖乳杆菌在治疗脂质紊乱中的新的潜在作用。
The microbiome plays an important role in lipid metabolism but how the introduction of probiotic communities affects host lipid metabolism is poorly understood. Using a multidisciplinary approach we addressed this knowledge gap using the zebrafish model by coupling high-throughput sequencing with biochemical, molecular and morphological analysis to evaluate the changes in the intestine. Analysis of bacterial 16S libraries revealed that Lactobacillus rhamnosus was able to modulate the gut microbiome of zebrafish larvae, elevating the abundance of Firmicutes sequences and reducing the abundance of Actinobacteria. The gut microbiome changes modulated host lipid processing by inducing transcriptional down-regulation of genes involved in cholesterol and triglycerides metabolism (fit2, agpat4, dgat2, mgll, hnf4α, scap, and cck) concomitantly decreasing total body cholesterol and triglyceride content and increasing fatty acid levels. L. rhamnosus treatment also increased microvilli and enterocyte lengths and decreased lipid droplet size in the intestinal epithelium. These changes resulted in elevated zebrafish larval growth. This integrated system investigation demonstrates probiotic modulation of the gut microbiome, highlights a novel gene network involved in lipid metabolism, provides an insight into how the microbiome regulates molecules involved in lipid metabolism, and reveals a new potential role for L. rhamnosus in the treatment of lipid disorders.
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影响因子：
29
作者：
Donohoe DR;Garge N;Zhang X;Sun W;O'Connell TM;Bunger MK;Bultman SJ
通讯作者：
Bultman SJ
影响因子：
3.5
作者：
De Rodriganez, M. A. Saenz;Diaz-Rosales, P.;Moyano, F. J.
通讯作者：
Moyano, F. J.
影响因子：
5.9
作者：
FAITH, DP
通讯作者：
FAITH, DP
DOI：
10.1016/0005-2760(86)90286-9
发表时间：
1986-04-15
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FREDRIKSON, G;TORNQVIST, H;BELFRAGE, P
通讯作者：
BELFRAGE, P
DOI：
10.1093/bioinformatics/btp636
发表时间：
2010-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Caporaso JG;Bittinger K;Bushman FD;DeSantis TZ;Andersen GL;Knight R
通讯作者：
Knight R