Gradient light interference microscopy for 3D imaging of unlabeled specimens.
Gradient light interference microscopy for 3D imaging of unlabeled specimens.
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DOI：
10.1038/s41467-017-00190-7
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发表时间：
2017-08-08
影响因子：
16.6
通讯作者：
Popescu G
中科院分区：
文献类型：
--
作者：
Nguyen TH;Kandel ME;Rubessa M;Wheeler MB;Popescu G
多次散射限制了厚标本光学成像的对比度。在这里，我们提出梯度光干涉显微镜（GLIM）从薄和厚未标记的标本中提取三维信息。GLIM利用一种特殊的低相干干涉测量技术从样品中提取相位信息，从而可以用于测量细胞质量、体积、表面积及其随时间的演变。因为它结合了多个强度图像，对应于两个干涉波之间的控制相移，梯度光干涉显微镜是能够抑制非相干背景由于多次散射。GLIM可能成为体外受精的一种有价值的工具，造影剂和荧光团可能会影响胚胎的生存能力。由于GLIM是作为现有倒置显微镜的附加模块实现的，我们预计它将迅速被生物界采用。生物成像的挑战包括标记、光漂白和光毒性以及光散射。在这里，Nguyen等人开发了一种定量相位方法，该方法使用低相干干涉测量法在散射组织中进行无标记3D成像。
Multiple scattering limits the contrast in optical imaging of thick specimens. Here, we present gradient light interference microscopy (GLIM) to extract three-dimensional information from both thin and thick unlabeled specimens. GLIM exploits a special case of low-coherence interferometry to extract phase information from the specimen, which in turn can be used to measure cell mass, volume, surface area, and their evolutions in time. Because it combines multiple intensity images that correspond to controlled phase shifts between two interfering waves, gradient light interference microscopy is capable of suppressing the incoherent background due to multiple scattering. GLIM can potentially become a valuable tool for in vitro fertilization, where contrast agents and fluorophores may impact the viability of the embryo. Since GLIM is implemented as an add-on module to an existing inverted microscope, we anticipate that it will be adopted rapidly by the biological community. Challenges in biological imaging include labeling, photobleaching and phototoxicity, as well as light scattering. Here, Nguyen et al. develop a quantitative phase method that uses low-coherence interferometry for label-free 3D imaging in scattering tissue.
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影响因子：
48
作者：
Godin, Michel;Delgado, Francisco Feijo;Son, Sungmin;Grover, William H.;Bryan, Andrea K.;Tzur, Amit;Jorgensen, Paul;Payer, Kris;Grossman, Alan D.;Kirschner, Marc W.;Manalis, Scott R.
通讯作者：
Manalis, Scott R.
影响因子：
27.1
作者：
Bhaduri, Basanta;Edwards, Chris;Popescu, Gabriel
通讯作者：
Popescu, Gabriel
影响因子：
2.5
作者：
Halvaei, Iman;Khalili, Mohammad Ali;Nottola, Stefania A.
通讯作者：
Nottola, Stefania A.
影响因子：
46.9
作者：
Hoebe, R. A.;Van Oven, C. H.;Manders, E. M. M.
通讯作者：
Manders, E. M. M.
影响因子：
17.1
作者：
Kandel, Mikhail E.;Teng, Kai Wen;Popescu, Gabriel
通讯作者：
Popescu, Gabriel