Genome-wide transcriptome analysis in murine neural retina using high-throughput RNA sequencing.
Genome-wide transcriptome analysis in murine neural retina using high-throughput RNA sequencing.
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DOI：
10.1016/j.ygeno.2011.09.003
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发表时间：
2012-01
期刊：
影响因子：
4.4
通讯作者：
Morrow, Eric M.
中科院分区：
文献类型：
--
作者：
Gamsiz, Ece D.;Ouyang, Qing;Schmidt, Michael;Nagpal, Shailender;Morrow, Eric M.
视网膜转录组的全基因组表征对于理解视觉系统的发育、生理和疾病至关重要。mRNA文库的大规模平行短读测序用于生成成年鼠神经视网膜的转录组的广泛图谱。RNA-seq数据有力地证实了先前通过微阵列和SAGE进行的转录组研究。然而，视网膜转录组的几个新特征被发现。例如，视网膜疾病基因被发现是转录组中表达最高的基因之一。我们还证明了视网膜转录组的其他有趣的功能，例如，视网膜似乎采用了一个非常具体和有限的一组突触囊泡基因，也有持久性的表达的大多数“神经发育”基因到成年期。利用新的测序方法进行视网膜转录组研究具有很高的信息量，这些数据也可以作为研究人员社区的资源。
Genome-wide characterization of the retinal transcriptome is central to understanding development, physiology and disorders of the visual system. Massively parallel, short-read sequencing of mRNA libraries was used to generate an extensive map of the transcriptome of the adult, murine neural retina. RNA-seq data strongly corroborates prior transcriptome studies by microarray and SAGE. However, several novel features of the retinal transcriptome were discovered. For example, retinal disease genes were discovered to be among the most highly expressed in the transcriptome. We also demonstrate other interesting features of the retinal transcriptome, for example, that the retina appears to employ a very specific and restricted set of synaptic vesicle genes, and also that there is persistence of expression of a majority of “neurodevelopmental” genes into adulthood. Retina transcriptome studies utilizing novel sequencing methods have been highly informative and these data may also serve as a resource for the community of researchers.
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影响因子：
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作者：
Zhang SS;Xu X;Liu MG;Zhao H;Soares MB;Barnstable CJ;Fu XY
通讯作者：
Fu XY
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
GOEAST：一个基于网络的软件工具包，用于基因本体富集分析。
影响因子：
14.9
作者：
Zheng Q;Wang XJ
通讯作者：
Wang XJ
DOI：
10.1038/nrg2934
发表时间：
2011-02
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
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影响因子：
4.8
作者：
Haeseleer, F;Sokal, I;Palczewski, K
通讯作者：
Palczewski, K