Alterations in mucosa-associated microbiota in the stomach of patients with gastric cancer.
Alterations in mucosa-associated microbiota in the stomach of patients with gastric cancer.
复制标题
胃癌患者胃粘膜相关微生物群的变化
DOI：
10.1007/s13402-021-00596-y
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发表时间：
2021-06
期刊：
影响因子：
--
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Deng Y;Ding X;Song Q;Zhao G;Han L;Ding B;Wang X;Hao X;Li H
目的本研究的目的是描述胃癌进展过程中胃不同解剖位置的粘膜相关微生物群的变化，并确定胃癌患者幽门螺杆菌感染和胃微生物变化之间的关系。幽门阴性的慢性胃炎患者和34例胃癌患者，包括H.幽门阴性和阳性的胃窦和胃体肿瘤患者。胃粘膜相关的微生物群测定16S核糖体RNA基因测序使用454测序platform.ResultsWe发现，从三个不同的解剖部位慢性胃炎的个人表现出不同的微生物群组成，虽然微生物的α多样性，丰富度和β多样性是相似的。与慢性胃炎患者相比，胃癌患者胃窦和胃体部的胃肠道菌群组成在有序水平上存在显著差异，且与H.幽门感染状态。微生物α多样性和物种丰富度，然而，慢性胃炎和胃癌病例之间是相似的，独立于H。幽门状态胃癌患者与慢性胃炎患者的微生物群落结构不同。此外，我们还发现H. pylorimarkedly改变了胃体癌的结构，但只有轻微的影响antrum.ConclusionOur数据显示，从胃炎到胃癌的进展过程中，不同的生态位特异性微生物群的改变。这些变化可能反映了微生物群对胃中不同特定环境栖息地的适应，并可能在胃癌发生中发挥重要但尚未确定的作用。
PurposeThe purpose of this study was to characterize alterations in mucosa-associated microbiota in different anatomical locations of the stomach during gastric cancer progression and to identify associations betweenHelicobacterpylori infection and gastric microbial changes in patients with gastric cancer.MethodsTwenty-fiveH. pylorinegative subjects with chronic gastritis and thirty-four subjects with gastric cancer were recruited, includingH. pylorinegative and positive patients with tumors in the antrum and the corpus. Gastric mucosa-associated microbiota were determined by 16S ribosomal RNA gene sequencing using a 454 sequencing platform.ResultsWe found that individuals with chronic gastritis from three different anatomical sites exhibited different microbiota compositions, although the microbial alpha diversity, richness and beta diversity were similar. Compared to patients with chronic gastritis, the gastric microbiota compositions were significantly different at the order level in the antrum and the corpus of patients with gastric cancer, which was dependent on theH. pyloriinfection status. Microbial alpha diversity and species richness, however, were similar between chronic gastritis and gastric cancer cases and independent ofH. pyloristatus. The microbial community structure in patients with gastric cancer was distinct from that in patients with chronic gastritis. In addition, we found that the presence ofH. pylorimarkedly altered the structure in gastric corpus cancer, but only mildly affected the antrum.ConclusionOur data revealed distinct niche-specific microbiota alterations during the progression from gastritis to gastric cancer. These alterations may reflect adaptions of the microbiota to the diverse specific environmental habitats in the stomach, and may play an important, as yet undetermined, role in gastric carcinogenesis.
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影响因子：
12.3
作者：
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通讯作者：
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