Facile Synthesis of Uniform Virus-like Mesoporous Silica Nanoparticles for Enhanced Cellular Internalization.
Facile Synthesis of Uniform Virus-like Mesoporous Silica Nanoparticles for Enhanced Cellular Internalization.
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均匀病毒样介孔二氧化硅纳米颗粒的便捷合成，可增强细胞内在化。
DOI：
10.1021/acscentsci.7b00257
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发表时间：
2017-08-23
影响因子：
18.2
通讯作者：
Zhao D
中科院分区：
文献类型：
--
作者：
Wang W;Wang P;Tang X;Elzatahry AA;Wang S;Al-Dahyan D;Zhao M;Yao C;Hung CT;Zhu X;Zhao T;Li X;Zhang F;Zhao D
目前纳米颗粒的细胞摄取效率低，极大地限制了其在生物医学领域的应用。在此，我们证明了新的病毒样介孔二氧化硅纳米粒子可以很容易地合成，显示出非常优越的上级细胞摄取性能。在低浓度表面活性剂的油/水两相体系中，通过单胶束外延生长法成功合成了具有粗糙表面的病毒状介孔二氧化硅纳米粒子。病毒样纳米颗粒的粗糙表面形貌主要来自于通过外延生长过程自发生长的介孔二氧化硅纳米管。所得纳米粒子显示出均匀的粒径和优异的单分散性。纳米颗粒的结构参数可以很好地调节，具有可控的核直径（160 -160 nm）、管状长度（166 -70 nm）和外径（166 -10 nm）。由于仿生形态，病毒样纳米颗粒显示出非常上级的细胞摄取特性（在几分钟内大量侵入活细胞，<5分钟），独特的内化途径和延长的血液循环持续时间（t1/2 = 2.16 h），这比传统的介孔二氧化硅纳米颗粒（0.45 h）长得多。此外，我们的外延生长策略可以应用于制造各种病毒样介孔核壳结构，为生物医学应用设计合成病毒样纳米复合材料铺平了道路。采用单胶束外延生长法成功合成了具有粗糙表面的病毒状介孔二氧化硅纳米粒子，并显示出优异的细胞摄取性能。
The low-efficiency cellular uptake property of current nanoparticles greatly restricts their application in the biomedical field. Herein, we demonstrate that novel virus-like mesoporous silica nanoparticles can easily be synthesized, showing greatly superior cellular uptake property. The unique virus-like mesoporous silica nanoparticles with a spiky tubular rough surface have been successfully synthesized via a novel single-micelle epitaxial growth approach in a low-concentration-surfactant oil/water biphase system. The virus-like nanoparticles’ rough surface morphology results mainly from the mesoporous silica nanotubes spontaneously grown via an epitaxial growth process. The obtained nanoparticles show uniform particle size and excellent monodispersity. The structural parameters of the nanoparticles can be well tuned with controllable core diameter (∼60–160 nm), tubular length (∼6–70 nm), and outer diameter (∼6–10 nm). Thanks to the biomimetic morphology, the virus-like nanoparticles show greatly superior cellular uptake property (invading living cells in large quantities within few minutes, <5 min), unique internalization pathways, and extended blood circulation duration (t1/2 = 2.16 h), which is much longer than that of conventional mesoporous silica nanoparticles (0.45 h). Furthermore, our epitaxial growth strategy can be applied to fabricate various virus-like mesoporous core–shell structures, paving the way toward designed synthesis of virus-like nanocomposites for biomedicine applications. Virus-like mesoporous silica nanoparticles with spiky tubular rough surface have been successfully synthesized via a novel single-micelle epitaxial growth approach, showing excellent cellular uptake property.
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影响因子：
18.2
作者：
Ahmad Nor Y;Niu Y;Karmakar S;Zhou L;Xu C;Zhang J;Zhang H;Yu M;Mahony D;Mitter N;Cooper MA;Yu C
通讯作者：
Yu C
影响因子：
15
作者：
BECK, JS;VARTULI, JC;SCHLENKER, JL
通讯作者：
SCHLENKER, JL
影响因子：
17.1
作者：
Albanese, Alexandre;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.
影响因子：
38.3
作者：
Jiang, Wen;Kim, Betty Y. S.;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.
影响因子：
15
作者：
Prencipe G;Tabakman SM;Welsher K;Liu Z;Goodwin AP;Zhang L;Henry J;Dai H
通讯作者：
Dai H