Exercise rather than fluoxetine promotes oligodendrocyte differentiation and myelination in the hippocampus in a male mouse model of depression.
Exercise rather than fluoxetine promotes oligodendrocyte differentiation and myelination in the hippocampus in a male mouse model of depression.
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在抑郁症雄性小鼠模型中，运动而非氟西汀可促进海马少突胶质细胞分化和髓鞘形成
DOI：
10.1038/s41398-021-01747-3
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发表时间：
2021-12-08
影响因子：
6.8
通讯作者：
Tang Y
中科院分区：
文献类型：
--
作者：
Tang J;Liang X;Dou X;Qi Y;Yang C;Luo Y;Chao F;Zhang L;Xiao Q;Jiang L;Zhou C;Tang Y
尽管选择性5-羟色胺再摄取抑制剂(SSRI)系统与情绪障碍的临床现象有意义地联系在一起，但15%-35%的患者对多次SSRI干预没有反应，甚至经历了病情的恶化。正如我们之前所展示的，跑步锻炼和氟西汀都能逆转类似抑郁的行为。然而，运动是否比氟西汀治疗更快地逆转抑郁样行为，以及这种快速效果是否通过促进海马区少突胶质细胞分化和/或髓鞘形成而实现，此前尚不清楚。本研究选用雄性C57BL/6 J小鼠60只。我们让具有不可预测的慢性应激的小鼠进行为期4周的跑步运动试验(ucs + RN)或给它们腹腔注射氟西汀(ucs + flx)来解决这些不确定性。在行为学水平上，第7周末，在蔗糖偏好试验中， + RN组小鼠的糖水摄入量明显高于 + 组；第8周末，UCSFlx组小鼠的糖水摄入量显著高于USC组。无偏见的体视学结果和免疫荧光分析表明，运动而不是氟西汀治疗增加了暴露于UCS的抑郁小鼠CA1亚区CC1+和CC1+/Orig2+/BrdU+少突胶质细胞的数量。此外，与氟西汀相比，跑步运动增加了有髓小鼠CA1区髓鞘碱性蛋白(MBP)的水平和有髓神经纤维的G-比率。与氟西汀不同的是，运动促进了海马髓鞘形成和少突胶质细胞分化，因此有可能作为一种治疗策略来减少由ucs诱导的抑郁样行为。
Although selective serotonin reuptake inhibitor (SSRI) systems have been meaningfully linked to the clinical phenomena of mood disorders, 15–35% of patients do not respond to multiple SSRI interventions or even experience an exacerbation of their condition. As we previously showed, both running exercise and fluoxetine reversed depression-like behavior. However, whether exercise reverses depression-like behavior more quickly than fluoxetine treatment and whether this rapid effect is achieved via the promotion of oligodendrocyte differentiation and/or myelination in the hippocampus was previously unknown. Sixty male C57BL/6 J mice were used in the present study. We subjected mice with unpredictable chronic stress (UCS) to a 4-week running exercise trial (UCS + RN) or intraperitoneally injected them with fluoxetine (UCS + FLX) to address these uncertainties. At the behavioral level, mice in the UCS + RN group consumed significantly more sugar water in the sucrose preference test (SPT) at the end of the 7th week than those in the UCS group, while those in the UCS + FLX group consumed significantly more sugar water than mice in the UCS group at the end of the 8th week. The unbiased stereological results and immunofluorescence analyses revealed that running exercise, and not fluoxetine treatment, increased the numbers of CC1+ and CC1+/Olig2+/BrdU+ oligodendrocytes in the CA1 subfield in depressed mice exposed to UCS. Moreover, running exercise rather than fluoxetine increased the level of myelin basic protein (MBP) and the G-ratio of myelinated nerve fibers in the CA1 subfield in the UCS mouse model. Unlike fluoxetine, exercise promoted hippocampal myelination and oligodendrocyte differentiation and thus has potential as a therapeutic strategy to reduce depression-like behaviors induced by UCS.
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影响因子：
11
作者：
Cao B;Passos IC;Mwangi B;Amaral-Silva H;Tannous J;Wu MJ;Zunta-Soares GB;Soares JC
通讯作者：
Soares JC
影响因子：
5
作者：
Costa Monteiro, Heloisa Mirelle;Barreto-Silva, Nathalia Lima;Amancio-dos-Santos, Angela
通讯作者：
Amancio-dos-Santos, Angela
影响因子：
5.3
作者：
Liu, Jia;Dupree, Jeffrey L.;Casaccia, Patrizia
通讯作者：
Casaccia, Patrizia
影响因子：
4.7
作者：
Gobinath, Aarthi R.;Richardson, Robin J.;Galea, Liisa A. M.
通讯作者：
Galea, Liisa A. M.
影响因子：
10.5
作者：
Cole, James;Chaddock, Christopher A.;Fu, Cynthia H. Y.
通讯作者：
Fu, Cynthia H. Y.