Characterization of opiate-mediated responses of the feline ileum and ileocecal sphincter.
Characterization of opiate-mediated responses of the feline ileum and ileocecal sphincter.
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猫回肠和回盲部括约肌阿片介导反应的表征。
DOI：
10.1172/jci110476
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Cohen,S
中科院分区：
文献类型：
--
作者：
Ouyang,A;Clain,CJ;SnapeJr,WJ;Cohen,S
虽然阿片肽已被证明在猫的肠道化学，这些肽的行动是未知的。本研究的目的是：（a）确定远端回肠和回盲部括约肌（ICS）对硫酸吗啡（MS）、甲硫氨酸脑啡肽（ME）和亮氨酸脑啡肽（LE）的反应：（B）确定外源性阿片类药物介导这些反应的机制;（c）确定参与介导这些反应的受体类型;以及（d）确定内源性阿片剂是否-介导的反应可以是迷走神经诱导的。ICS对所有三种阿片受体激动剂均产生强直性和阶段性收缩，后者与峰电位活动增加相关。ICS压力反应的EDmax对于ME为1 μg/kg，对于LE为5 μg/kg，对于MS为150 μg/kg。远端回肠以增加的峰电位活动和阶段性收缩响应。ME、LE和MS的回肠运动指数反应的EDmax分别为1.0 × 10−1μg/kg、1 μg/kg和150 μg/kg。因此，两个部位表现出相似的剂量-反应关系，均对比MS低至少100倍的脑啡肽剂量产生反应。ICS收缩先于回肠收缩。阿托品、六甲铵、酚妥拉明、普萘洛尔、肉桂色林或河豚毒素均不能拮抗回肠和ICS反应。纳洛酮（600 μg/kg）可拮抗脑啡肽引起的收缩反应，而纳洛酮（10 μg/kg）可拮抗MS引起的收缩反应。在引起收缩反应之前，需要更高剂量的特异性受体激动剂SKF 10047和κ受体激动剂酮环唑辛。电刺激颈部迷走神经引起ICS收缩和血压下降。纳洛酮1 mg/kg可抑制ICS的收缩反应，但对血压反应无影响。这些数据表明：（a）强直性和阶段性ICS收缩，随后是回肠收缩，可能是通过位于肌膜中的δ-型阿片受体介导的，（B）阿片介导的ICS收缩可能在迷走神经刺激期间诱导。
Although opioid peptides have been demonstrated immunohistochemically in the feline intestine, the action of these peptides is unknown. The aims of this study were: (a) to determine the distal ileal and ileocecal sphincter (ICS) responses to morphine sulfate (MS), methionine enkephalin (ME) and leucine enkephalin (LE); (b) to determine the mechanism by which exogenous opiates mediate these responses; (c) to determine the type of receptor involved in mediating these responses and (d) to ascertain whether endogenous opiate-mediated responses may be vagally induced. The ICS responded to all three opiate agonists with tonic and phasic contractions, the latter being associated with increased spike activity. The EDmaxfor ICS pressure response was 1 μg/kg for ME, 5 μg/kg for LE, and 150 μg/kg for MS. The distal ileum responded with increased spike activity and phasic contractions. The EDmaxfor the ileal motility index response was 1.0 × 10−1μg/kg for ME, 1 μg/kg for LE, and 150 μg/kg for MS. Thus, both sites demonstrated similar dose-response relationships, both responding to at least 100 times lower doses of enkephalins than MS. The ICS contraction preceded ileal contractions. The ileal and ICS response was not antagonized by atropine, hexamethonium, phentolamine, propranolol, cinanserin, or tetrodotoxin. Naloxone, 600 μg/kg, antagonized the response to the enkephalins while 10 μg/kg antagonized the response to MS. Higher doses of the specific-receptor agonist SKF 10047 and κ-receptor agonist ketocyclazocine were required before a contractile response was elicited. Electrical stimulation of the cervical vagus induced ICS contraction and a fall in blood pressure. The ICS contractile response but not the blood pressure response was inhibited by naloxone 1 mg/kg. These data indicate: (a) tonic and phasic ICS contraction followed by ileal contraction may be mediated through δ-type opiate receptors located in the muscle membrane and (b) opiate-mediated ICS contraction may be induced during vagal stimulation.
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影响因子：
64.8
作者：
HUGHES, J;SMITH, TW;MORRIS, HR
通讯作者：
MORRIS, HR
禁食和呕吐时猫十二指肠的电活动。
影响因子：
29.4
作者：
N. Weisbrodt;J. Christensen
通讯作者：
J. Christensen
DOI：
10.1016/s0140-6736(77)92277-2
发表时间：
1977
期刊：
The Lancet
影响因子：
--
作者：
J. Polak;S. Bloom;S. Sullivan;P. Facer;A. Pearse
通讯作者：
A. Pearse
吗啡对大鼠肠道蠕动的急性影响。
影响因子：
5
作者：
T. Burks
通讯作者：
T. Burks
迷走神经脑啡肽能神经控制猫幽门和胃的证据。
影响因子：
29.4
作者：
R. Edin;J. Lundberg;L. Terenius;A. Dahlström;T. Hökfelt;J. Kewenter;H. Ahlman
通讯作者：
H. Ahlman