Evidence that a phage T4 DNA packaging enzyme is a processed form of the major capsid gene product.

Evidence that a phage T4 DNA packaging enzyme is a processed form of the major capsid gene product.
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有证据表明噬菌体 T4 DNA 包装酶是主要衣壳基因产物的加工形式。

DOI:
10.1016/0092-8674(85)90293-4
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发表时间:
1985
期刊:
影响因子:
64.5
通讯作者:
Black,LW
Black,LW
中科院分区:
生物学1区
文献类型:
--
作者:
Rao,VB;Black,LW

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噬菌体 T4 DNA 包装酶似乎是作为主要 T4 衣壳结构蛋白 gp23 的加工形式出现的。所有头基因突变体均缺失酶活性和抗原,从而阻断体内头蛋白的形态发生蛋白水解加工反应。通过对含有 gp23 的提取物进行 T4 (gp21) 特异性处理,可以在体外形成酶抗原。酶抗原存在于活性加工的前头部中,但未存在于完整头部中。该酶和主要衣壳蛋白表现出免疫交叉反应性,在蛋白水解后产生常见的肽,并共享组装构象依赖性 ATP 结合位点。包装酶和成熟衣壳蛋白 (gp23') 似乎均源自 gp23 的加工,前者是前头中特定 gp23 结构的次要产物,在 DNA 包装中作为 DNA 依赖性 ATP 酶和头依赖性终止酶发挥作用。
A phage T4 DNA packaging enzyme appears to arise as a processed form of the major T4 capsid structural protein gp23. The enzyme activity and antigen are missing from all head gene mutants that block the morphogenetic proteolytic processing reactions of the head proteins in vivo. The enzyme antigen can be formed in vitro by T4 (gp21) specific processing of gp23 containing extracts. Enzyme antigen is found in active processed proheads but not in full heads. The enzyme and the major capsid protein show immunological cross-reactivity, produce common peptides upon proteolysis, and share an assembly-conformation-dependent ATP binding site. The packaging enzyme and the mature capsid protein (gp23’) both appear to arise from processing of gp23, the former as a minor product of a specific gp23 structure in the prohead, acting in DNA packaging as a DNA-dependent ATPase, and a headful-dependent terminase.
鸡骨骼肌体内和体外肌球蛋白重链和轻链亚型的发育表现。
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