Broad-range survey of vector-borne pathogens and tick host identification of Ixodes ricinus from Southern Czech Republic.
Broad-range survey of vector-borne pathogens and tick host identification of Ixodes ricinus from Southern Czech Republic.
复制标题
DOI：
10.1093/femsec/fix129
复制
发表时间：
2017-11-01
影响因子：
4.2
通讯作者：
Eshoo MW
中科院分区：
文献类型：
--
作者：
Honig V;Carolan HE;Vavruskova Z;Massire C;Mosel MR;Crowder CD;Rounds MA;Ecker DJ;Ruzek D;Grubhoffer L;Luft BJ;Eshoo MW
蓖麻硬蜱是许多人类和动物病原体的载体。它们是宿主多面手，能够以 300 多种脊椎动物为食。蜱传病原体的流行受到宿主-媒介-病原体相互作用的影响，从而导致感染风险的空间分布。采用广谱聚合酶链反应电喷雾电离质谱 (PCR/ESI-MS) 对捷克共和国南部四个地点的 435 只蓖麻若虫进行分析，以对蜱传病原体进行物种鉴定。伯氏疏螺旋体螺旋体是蜱中最常见的病原体； 21% 的蜱对单一基因种呈阳性，2% 的蜱同时感染两种基因种。其他检测到的蜱传病原体包括瑞士立克次体 (3.9%)、莫纳森立克次体 (0.2%)、嗜吞噬细胞无形体 (2.8%)、猎性巴贝斯虫 (0.9%) 和巴贝斯虫 (0.9%)。微型（0.5%）。蜱虫的脊椎动物宿主通过PCR和蜱虫血粉残留物的反向线印迹杂交来确定。 61% 的蜱虫的宿主已被确定。在成功识别宿主的样本中，16% 的样本中检测到了两种宿主的 DNA。大多数蜱以偶蹄类动物（50.7%）为食，其次是啮齿类动物（28.6%）和鸟类（7.8%）。其他宿主物种包括野猪、鹿、松鼠、田鼠和田鼠。对寻找宿主的蓖麻硬蜱若虫中多种人类和兽医病原体的流行率进行了估计，并确定了它们以前的脊椎动物宿主。
Ixodes ricinus ticks are vectors of numerous human and animal pathogens. They are host generalists able to feed on more than 300 vertebrate species. The prevalence of tick-borne pathogens is influenced by host–vector–pathogen interactions that results in spatial distribution of infection risk. Broad-range polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was used to analyze 435 I. ricinus nymphs from four localities in the south of the Czech Republic for the species identification of tick-borne pathogens. Borrelia burgdorferi sensu lato spirochetes were the most common pathogen detected in the ticks; 21% of ticks were positive for a single genospecies and 2% were co-infected with two genospecies. Other tick-borne pathogens detected included Rickettsia helvetica (3.9%), R. monacensis (0.2%), Anaplasma phagocytophilum (2.8%), Babesia venatorum (0.9%), and Ba. microti (0.5%). The vertebrate host of the ticks was determined using PCR followed by reverse line blot hybridization from the tick's blood-meal remnants. The host was identified for 61% of ticks. DNA of two hosts was detected in 16% of samples with successful host identification. The majority of ticks had fed on artiodactyls (50.7%) followed by rodents (28.6%) and birds (7.8%). Other host species were wild boar, deer, squirrels, field mice and voles. Prevalence of multiple human and veterinary pathogens was estimated in host-seeking nymphal Ixodes ricinus ticks, and their previous vertebrate host was determined.
登录
查看更多内容
影响因子：
11.8
作者：
Crowder CD;Carolan HE;Rounds MA;Honig V;Mothes B;Haag H;Nolte O;Luft BJ;Grubhoffer L;Ecker DJ;Schutzer SE;Eshoo MW
通讯作者：
Eshoo MW
影响因子：
2.1
作者：
Burri, C.;Bastic, V.;Gern, L.
通讯作者：
Gern, L.
影响因子：
3.7
作者：
Crowder, Chris D.;Matthews, Heather E.;Eshoo, Mark W.
通讯作者：
Eshoo, Mark W.
DOI：
10.1016/j.cimid.2016.06.003
发表时间：
2016-08-01
影响因子：
2
作者：
Ebani, Valentina Virginia;Rocchigiani, Guido;Mancianti, Francesca
通讯作者：
Mancianti, Francesca
影响因子：
2.8
作者：
Bunikis, J;Garpmo, U;Barbour, AG
通讯作者：
Barbour, AG