3D-Printed pHEMA Materials for Topographical and Biochemical Modulation of Dorsal Root Ganglion Cell Response.
3D-Printed pHEMA Materials for Topographical and Biochemical Modulation of Dorsal Root Ganglion Cell Response.
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DOI：
10.1021/acsami.7b06742
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发表时间：
2017-09-13
影响因子：
9.5
通讯作者：
Nuzzo RG
中科院分区：
文献类型：
--
作者：
Badea A;McCracken JM;Tillmaand EG;Kandel ME;Oraham AW;Mevis MB;Rubakhin SS;Popescu G;Sweedler JV;Nuzzo RG
理解和控制细胞和工程材料之间发生的相互作用是生物医学设备发展进程中的核心挑战。在这项工作中，我们描述了用于直接墨水书写（DIW）的材料，这是一种基于挤压的3D打印类型，它在3D水凝胶支架的微丝内嵌入定制合成蛋白（RGD-PDL），以改变这些相互作用，并在体外以差异方式直接复杂细胞群的组织水平组织。RGD-PDL是用含有整合素结合基序Arg-Gly-Asp （RGD）的肽段对聚d -赖氨酸（PDL）进行不同程度的修饰而合成的。RGD-PDL由图图化和薄膜聚（2-羟乙基）甲基丙烯酸酯（pHEMA）底物呈现的成分梯度允许以灰度形式绘制细胞生长依从性的图图化。使用模型NIH-3T3成纤维细胞系证明了rgd - pdl修饰的pHEMA材料对细胞生长的表面化学依赖性指导。在这些支架上也可以形成更复杂的细胞系统——器官型原代小鼠背根神经节（DRG），根据其RGD-PDL含量和地形，可以看到不同形式的细胞生长和迁移指导。该实验平台用于研究有机型培养物形成和重组的理化因素，为组织工程、再生医学和诊断学的研究提供了有用的能力。
Understanding and controlling the interactions occurring between cells and engineered materials are central challenges towards progress in the development of biomedical devices. In this work, we describe materials for direct ink writing (DIW), an extrusion-based type of 3D printing, that embed a custom synthetic protein (RGD-PDL) within the microfilaments of 3D-hydrogel scaffolds to modify these interactions and differentially direct tissue-level organization of complex cell populations in vitro. The RGD-PDL is synthesized by modifying poly-D-lysine (PDL) to varying extents with peptides containing the integrin-binding motif Arg-Gly-Asp (RGD). Compositional gradients of the RGD-PDL presented by both patterned and thin-film poly-(2-hydroxyethyl) methacrylate (pHEMA) substrates allow the patterning of cell-growth compliance in a grayscale form. The surface chemistry-dependent guidance of cell growth on the RGD-PDL-modified pHEMA materials is demonstrated using a model NIH-3T3 fibroblast cell line. The formation of a more complex cellular system — organotypic primary murine dorsal root ganglion (DRG) – in culture is also achieved on these scaffolds, where distinctive forms of cell growth and migration guidance are seen depending on their RGD-PDL content and topography. This experimental platform for the study of physicochemical factors on the formation and the reorganization of organotypic cultures offers useful capabilities for studies in tissue engineering, regenerative medicine, and diagnostics.
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影响因子：
2.8
作者：
Clarke, Joseph C.;Tuft, Bradley W.;Clinger, John D.;Levine, Rachel;Figueroa, Lucas Sievens;Guymon, C. Allan;Hansen, Marlan R.
通讯作者：
Hansen, Marlan R.
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影响因子：
41.2
作者：
Gladman, A. Sydney;Matsumoto, Elisabetta A.;Lewis, Jennifer A.
通讯作者：
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影响因子：
3.8
作者：
Adams, Robert D.;Rendell, Sara R.;Harkins, Amy B.
通讯作者：
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