Traffic jam regulates the function of the ovarian germline stem cell progeny differentiation niche during pre-adult stage in Drosophila
Traffic jam regulates the function of the ovarian germline stem cell progeny differentiation niche during pre-adult stage in Drosophila
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交通堵塞调节果蝇成体前期卵巢生殖干细胞后代分化生态位的功能
DOI：
10.1038/s41598-019-45317-6
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发表时间：
2019-07
影响因子：
4.6
通讯作者：
Lei Zhang
中科院分区：
文献类型：
--
作者：
Mengjie Li;Xiaolong Hu;Shu Zhang;Margaret S. Ho;Geng Wu;Lei Zhang
干细胞自我更新和子细胞分化受到各自的小生境的严格调节，这些小生境产生外部线索以支持适当的发育。在果蝇卵巢中，Dpp从生殖系干细胞（GSC）龛中分泌，并激活GSC中的BMP信号以进行自我更新。分化龛中的护送细胞（EC）限制GSC龛外的Dpp并延伸突起以帮助GSC子细胞的适当分化。在这里，我们提供的证据表明，大Maf转录因子交通堵塞（Tj）块GSC后代分化的损失。时空特异性敲低实验表明，Tj是需要在成体前EC谱系的生殖细胞分化控制。进一步的分子和遗传学分析表明，tj缺失导致的种系分化缺陷部分归因于分化生态位中dpp的升高。此外，我们的研究表明，tj-耗竭诱导GSC生态位外的异位En表达，这有助于EC中的dpp表达上调以及GSC后代分化缺陷。或者，由tj-耗尽引起的EC突起的损失和EC数量的减少也可能部分地导致种系分化缺陷。总的来说，我们的研究结果表明，Tj在EC调节生殖细胞分化通过控制分化生态位的特点。
Stem cell self-renewal and the daughter cell differentiation are tightly regulated by the respective niches, which produce extrinsic cues to support the proper development. In Drosophila ovary, Dpp is secreted from germline stem cell (GSC) niche and activates the BMP signaling in GSCs for their self-renewal. Escort cells (ECs) in differentiation niche restrict Dpp outside the GSC niche and extend protrusions to help with proper differentiation of the GSC daughter cells. Here we provide evidence that loss of large Maf transcriptional factor Traffic jam (Tj) blocks GSC progeny differentiation. Spatio-temporal specific knockdown experiments indicate that Tj is required in pre-adult EC lineage for germline differentiation control. Further molecular and genetic analyses suggest that the defective germline differentiation caused by tj-depletion is partly attributed to the elevated dpp in the differentiation niche. Moreover, our study reveals that tj-depletion induces ectopic En expression outside the GSC niche, which contributes to the upregulated dpp expression in ECs as well as GSC progeny differentiation defect. Alternatively, loss of EC protrusions and decreased EC number elicited by tj-depletion may also partially contribute to the germline differentiation defect. Collectively, our findings suggest that Tj in ECs regulates germline differentiation by controlling the differentiation niche characteristics.
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影响因子：
4.6
作者：
Gancz, Dana;Gilboa, Lilach
通讯作者：
Gilboa, Lilach
护送细胞中的组蛋白 H1 缺陷引发果蝇卵巢生殖系肿瘤
影响因子：
2.7
作者：
Zhihao Yang;Jin Sun;Yuzhao Hu;Fang Wang;Xia Wang;Huan-Huan Qiao;Jiang Xu;Decai Mao;Xingjie Ren;Li-Xia Pan;Rong-Gang Xu;Bo-Wen Xu;Yifan Zhang;Haiyi Li;Wei Miao;Yanhui Hu;Zhijie Chang;Dong Wang;Haitao Li;Zai Chang;Lu-Ping Liu;Qingfei Liu;Jian-Quan Ni
通讯作者：
Jian-Quan Ni
影响因子：
44.1
作者：
Dániel Kirilly;T. Xie
通讯作者：
Dániel Kirilly;T. Xie
影响因子：
7.7
作者：
Wang S;Gao Y;Song X;Ma X;Zhu X;Mao Y;Yang Z;Ni J;Li H;Malanowski KE;Anoja P;Park J;Haug J;Xie T
通讯作者：
Xie T
影响因子：
4.6
作者：
Banisch, Torsten U.;Maimon, Iris;Gilboa, Lilach
通讯作者：
Gilboa, Lilach