Progranulin expression in neural stem cells and their differentiated cell lineages: an immunocytochemical study.
Progranulin expression in neural stem cells and their differentiated cell lineages: an immunocytochemical study.
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神经干细胞及其分化细胞谱系中颗粒体蛋白前体的表达：一项免疫细胞化学研究。
DOI：
10.3892/mmr.2013.1664
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发表时间：
2013-11
影响因子：
3.4
通讯作者：
KAIHUA GUO
中科院分区：
文献类型：
--
作者：
LANHAI LÜ;LI LUO;YINGHONG LU;LIZHI CHEN;JIE XU;KAIHUA GUO
颗粒体蛋白前体 (PGRN) 是一种神经营养因子，可调节神经突生长并增强神经元存活。 PGRN 和神经干细胞 (NSC) 分化之间的关联可能有助于阐明神经退行性疾病的潜在发病机制和潜在治疗方法。为了研究 PGRN 和 NSC 及其谱系之间的关联，从新生 Sprague Dawley 幼犬（年龄，1 天）的室下区制备原代 NSC，并在神经干细胞培养基中培养。培养 7 天后，NSC 聚集成神经球，并在传代 3 代后在差异培养基中维持 7 天。此外，通过免疫细胞化学分析了神经球和分化细胞（神经元、星形胶质细胞和少突胶质细胞）中的 PGRN 表达。还通过双重免疫荧光染色研究了新生大鼠（1 天和 7 天）脑组织中 PGRN 的表达和定位。数据表明，PGRN 在体外 NSC 及其分化细胞谱系中高表达。结果还表明，PGRN 在体内主要表达于神经元和小胶质细胞中，少量表达于 NSC、星形胶质细胞和少突胶质细胞中。本研究表明PGRN可能参与NSC分化的调节。
Progranulin (PGRN) is a neurotrophic factor that regulates neurite outgrowth and enhances neuronal survival. The association between PGRN and neural stem cell (NSC) differentiation may aid in elucidating the underlying pathogenesis and potential treatments for neurodegenerative diseases. To investigate the association between PGRN and NSCs and their lineages, primary NSCs were prepared from the subventricular zone of neonatal Sprague Dawley pups (age, 1 day) and cultured in the neural stem cell medium. After 7 days in culture, NSCs aggregated into neurospheres and were maintained in the differential medium for 7 days following three passages. In addition, PGRN expression in neurospheres and differentiated cells (neurons, astrocytes and oligodendrocytes) were analyzed by immunocytochemistry. The PGRN expression and localization was also investigated in the brain tissues of neonatal rats (age, 1 and 7 days) by double immunofluorescence staining. The data indicated that PGRN was highly expressed in NSCs and their differentiated cell lineages in vitro. The results also demonstrated that PGRN was predominantly expressed in neurons and microglia in vivo, and marginally in NSCs, astrocytes and oligodendrocytes. The present study suggested that PGRN may be involved in the regulation of NSC differentiation.
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影响因子：
3.4
作者：
Bergström T;Forsberg-Nilsson K
通讯作者：
Forsberg-Nilsson K
DOI：
10.1083/jcb.200712039
发表时间：
2008-04-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Van Damme P;Van Hoecke A;Lambrechts D;Vanacker P;Bogaert E;van Swieten J;Carmeliet P;Van Den Bosch L;Robberecht W
通讯作者：
Robberecht W
影响因子：
4.3
作者：
M. Yanagisawa;R. Yu
通讯作者：
M. Yanagisawa;R. Yu
影响因子：
4.2
作者：
Xu J;Xilouri M;Bruban J;Shioi J;Shao Z;Papazoglou I;Vekrellis K;Robakis NK
通讯作者：
Robakis NK
影响因子：
2.5
作者：
J. H. Park;M. Choi;Kyoung Sun Park;S. H. Kim;K. Jung;Y. Chai
通讯作者：
J. H. Park;M. Choi;Kyoung Sun Park;S. H. Kim;K. Jung;Y. Chai