Clonal spread of carbapenem non-susceptible Acinetobacter baumannii in an intensive care unit in a teaching hospital in China.
Clonal spread of carbapenem non-susceptible Acinetobacter baumannii in an intensive care unit in a teaching hospital in China.
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DOI：
10.3343/alm.2012.32.6.413
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发表时间：
2012-11
影响因子：
4.9
通讯作者：
Xu H
中科院分区：
文献类型：
--
作者：
Zhong Q;Xu W;Wu Y;Xu H
本研究旨在调查中国苏州某教学医院重症监护病房（ICU）医院感染病原体鲍曼不动杆菌的遗传多样性和耐药性。A.使用多位点序列分型（MLST）研究ICU中的鲍曼不动杆菌分离株。通过进行抗生素敏感性试验（包括琼脂稀释法和E-试验法）确定抗生素耐药性模式。耐药决定因素，例如，通过特异性PCR和DNA测序分析碳青霉烯酶基因、金属-β-内酰胺酶和1类整合子。在本研究中，33株非重复分离株被鉴定为5种现有序列类型（ST）（ST 92、ST 75、ST 112、ST 145和ST 345）和1种新序列类型ST n，其在ST 251的ropD 40上的nt 268处具有G-A突变。这些结果表明，碳青霉烯类非敏感A.鲍曼不动杆菌（CNSAb）分离株，其仅由2种不同的ST组成，其中ST 92和ST 75聚类成克隆复合物（CC）92。大多数CNSAb分离株（94.4%，17/18）携带OXA-23基因，而碳青霉烯类敏感的A.此外，66.7%（22/33）的鲍曼不动杆菌（CSAb）Ⅰ类整合酶阳性，基因盒分析显示其中有3个基因阵列，即，aacA 4-catB 8-aadA 1（77.3%，17/22）、aacA 4（22.7%，5/22）和aacC 1-orfX-orfX '-aadA 1（4.5%，1/22）。当所有这些数据相结合，抗生素耐药性和广泛分布的CNSAb分离在我们的ICU可能是由CC 92克隆的扩展。
This study was aimed to investigate the genetic diversity and antibiotic resistance profile of the nosocomial infection agent Acinetobacter baumannii from a medical intensive care unit (ICU) in a teaching hospital in Suzhou, China. The genetic relationship among A. baumannii isolates in an ICU was investigated using multilocus sequence typing (MLST). The antibiotic resistance pattern was determined by performing an antibiotic susceptible test, which included an agar dilution method and an E-test method. Resistant determinants, e.g., carbapenemase genes, metallo-β-lactamases, and class 1 integron, were analyzed by specific PCR and DNA sequencing. In the present study, 33 non-duplicate isolates were identified as 5 existing sequence types (STs) (ST92, ST75, ST112, ST145, and ST345) and 1 new sequence type STn, which has a G-A mutation at nt268 on ropD40 of ST251. These results reveal limited diversity in carbapenem non-susceptible A. baumannii (CNSAb) isolates in our ICU, which are comprised of only 2 distinct STs, with ST92 and ST75 clustering into a clonal complex (CC) 92. Most CNSAb isolates (94.4%, 17/18) harbored the OXA-23 gene, while no carbapenem-susceptible A. baumannii (CSAb) isolates harbored it. In addition, 66.7% (22/33) isolates were positive for class 1 integrase, and gene cassette analysis showed there are 3 gene arrays among them, i.e., aacA4-catB8-aadA1 (77.3%, 17/22), aacA4 (22.7%, 5/22), and aacC1-orfX-orfX'-aadA1 (4.5%, 1/22). When all these data are combined, the antibiotic resistance and wide distribution of CNSAb isolates in our ICU are probably caused by expansion of the CC92 clone.
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影响因子：
4.9
作者：
Poirel, L;Marqué, S;Nordmann, P
通讯作者：
Nordmann, P
影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
3.2
作者：
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通讯作者：
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