Turn-on fluorescent probe for dopamine detection in solutions and live cells based on in situ formation of aminosilane-functionalized carbon dots.
Turn-on fluorescent probe for dopamine detection in solutions and live cells based on in situ formation of aminosilane-functionalized carbon dots.
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DOI：
10.1016/j.aca.2021.338394
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发表时间：
2021-05-01
影响因子：
6.2
通讯作者：
Liao X
中科院分区：
文献类型：
--
作者：
Tang XY;Liu YM;Bai XL;Yuan H;Hu YK;Yu XP;Liao X
多巴胺（DA）是多种神经系统疾病的重要生物标志物。一种简单、快速的DA检测方法将为这些疾病的临床诊断和治疗提供帮助。在这项工作中，我们开发了一种新的无预处理的多巴胺检测方法，使用碳点作为原位合成的开启荧光探针。通过N-[3-（三甲氧基硅基）丙基]乙二胺（AEATMS）与多巴胺的温和缩合反应，制备了氨基硅烷功能化碳点（SiCDs），可直接用作多巴胺检测探针。SiCDs表现出绿色荧光，激发/发射最大值为380/495 nm。通过测量SiCDs的荧光强度，可以很容易地量化反应体系中DA的浓度。线性范围为0.1 ~ 100 μM，检出限为56.2 nM。该方法具有良好的选择性，加样回收率为101.77 ~ 119.91%，rsd为3.67%。我们还发现这些SiCDs可以在37℃的MN9D活细胞内合成，并产生明亮的荧光，这可以作为DA在细胞内分布的指示。所描述的方法在DA检测和活细胞成像方面具有简单、廉价和敏感的特点。以测试样品中的硅烷和多巴胺为原料原位合成绿色荧光碳点。荧光强度与样品中的多巴胺浓度相关。sicd可以在活细胞内合成，并指示多巴胺的分布。
Dopamine (DA) is a critical biomarker for a variety of neurological diseases. A simple and rapid DA detection method will provide aids for clinical diagnosis and treatments for those diseases. In this work, we developed a novel pretreatment-free method for dopamine detection using carbon dots as a turn-on fluorescent probe that was in situ synthesized. In a mild condensation reaction between N-[3-(Trimethoxysilyl)propyl]ethylenediamine (AEATMS) and dopamine, the aminosilane-functionalized carbon dots (SiCDs) were produced, which can be directly used as the dopamine detecting probe. SiCDs exhibited green fluorescence with excitation/emission maximum at 380/495 nm. By measuring the fluorescent intensity of SiCDs, DA concentrations in the reaction system were easily quantified. The linear range of the assay was between 0.1 to 100 μM with a limit of detection (LOD) of 56.2 nM. The probe is of good selectivity and the recoveries of the developed method were in the range of 101.77–119.91% with RSDs within 3.67% in human serum sample tests. We also found that these SiCDs can be synthesized within living MN9D cells under the 37 °C and generated bright fluorescence, which can serve as an indication for the distribution of DA in the cells. The described method exhibit potential in DA detection and live cell imaging for its feature of facility, inexpensiveness, and sensitivity. Green fluorescence emitting carbon dots were in situ synthesized from silane and dopamine in the testing samples. The fluorescent intensities were correlated to dopamine concentrations in the samples. SiCDs can be synthesized inside live cells and indicate the distribution of dopamine.
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影响因子：
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影响因子：
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通讯作者：
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