Development of a MD-LC-MS/MS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats
Development of a MD-LC-MS/MS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats
复制标题
活血止痛贴剂中 3 种生物活性化合物分析的 MD-LC-MS/MS 方法的建立及其在大鼠药代动力学研究中的应用
DOI：
10.1155/2019/6173565
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发表时间：
2019-01
影响因子：
--
通讯作者：
Liu Daofang
中科院分区：
文献类型：
--
作者：
Cui Yahua;Yang Chang;Liang Jun;Zhong Linying;Liu Caifeng;Bai Jie;Du Shouying;Liu Daofang
活血止痛橡皮膏是一种著名的中药方剂，用于治疗疼痛和炎症。在本研究中，采用了一种微透析-超高效液相色谱-串联质谱（MD - LC - MS/MS）方法
Huoxuezhitong rubber patch, a well-known traditional Chinese medicine (TCM) prescription, is utilized to treat pain and inflammation. In this study, a microdialysis-ultra-high-performance liquid chromatography-tandem mass spectrometry (MD-LC-MS/MS) method was designed for the simultaneous determination of active constituents in the rubber patch, such as paeonol (Pae), eugenol (Eug), and piperine (Pip). A microdialysis probe was implanted in the subcutaneous tissue of a rat, which is intended to detect the subcutaneous concentrations of target components. Saline containing 30% ethanol acted as perfusion fluid. Analytes in the microdialysate were completely separated over an ACQITY UPLC RBEH C18 column (2.1mm×100mm, 1.7μm). The mobile phase was composed of 0.01% ammonia aqueous and acetonitrile-0.01% ammonia with gradient elution. The single-run analysis time was 10.0 minutes. The linear regression displayed good linear relationships in the ranges of 0.25–100 ng/mL for paeonol and eugenol and 0.001–5 ng/mL for piperine. The interday and intraday precision of the quality control samples exhibited relative standard deviations (RSD) <13.56%. The accuracy values ranged from −14.92% to 14.00%. The present method was successfully applied in pharmacokinetics studies following dermal administration of Huoxuezhitong rubber patch in rats. Pip's Tmax (488.00±150.73) min was greater than that of Pae (186.67±48.44) min and Eug (240.00±138.56) min, and the rank order of t1/2 was Pae > Pip > Eug. The rank order of AUC0-720 and Cmax was both Eug > Pae > Pip. MRT0-∞ of Pip was higher than that of Pae and Eug. Eugenol showed a faster elimination and a shorter half-life. Paeonol showed a stronger drug reservoir function after removing the drug source.
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DOI：
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发表时间：
2003
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
--
作者：
W. Jia;Wen‐yuan Gao;Tao Wang;Yun-bin Liu;Jing Xue;P. Xiao
通讯作者：
W. Jia;Wen‐yuan Gao;Tao Wang;Yun-bin Liu;Jing Xue;P. Xiao
影响因子：
10.2
作者：
Yang M;Gu Y;Yang D;Tang X;Liu J
通讯作者：
Liu J
DOI：
10.4268/cjcmm20162226
发表时间：
2016-11
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
--
作者：
C. Yang;Jie Bai;S. Du;Yahua Cui;Qin-Shuai Zhang;Junming Ma
通讯作者：
C. Yang;Jie Bai;S. Du;Yahua Cui;Qin-Shuai Zhang;Junming Ma
影响因子：
4.3
作者：
Ying, Xiaozhou;Yu, Kehe;Peng, Lei
通讯作者：
Peng, Lei
影响因子：
2.9
作者：
Cilurzo, Francesco;Vistoli, Giulio;Minghetti, Paola
通讯作者：
Minghetti, Paola