Correlation of cell surface marker expression with African swine fever virus infection.
Correlation of cell surface marker expression with African swine fever virus infection.
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DOI：
10.1016/j.vetmic.2013.12.001
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发表时间：
2014-01-31
影响因子：
3.3
通讯作者：
Chapman D
中科院分区：
文献类型：
--
作者：
Lithgow P;Takamatsu H;Werling D;Dixon L;Chapman D
对非洲猪瘟病毒（ASFV）感染细胞表面标志物的表达进行了评价，以评估其与感染的关系。先前的研究结果表明，CD163表达与ASFV易感性相关。然而，在本研究中，猪CD163在细胞系上的表达并没有增加这些细胞的感染率，这表明在决定感染易感性方面，其他因素可能很重要。在贴壁猪骨髓细胞（pBM）上，CD45的表达与感染密切相关。CD163和CD203a的表达在中等水平上与感染相关，表明表达这些标记的细胞可能被感染，但不会优先被病毒感染。大多数表达MHCII的细胞被感染，表明它们可能被优先感染，尽管MHCII的表达不是感染所必需的，而且很大比例的感染细胞是MHCII阴性。CD16在感染后表达明显下降，感染细胞表达CD16的水平明显降低。总之，这些结果表明CD163可能参与ASFV感染，但可能不是必需的；该结果还强调了其他需要进一步研究的细胞标记的重要性。
The expression of surface markers on African swine fever virus (ASFV) infected cells was evaluated to assess their involvement in infection. Previous findings indicated CD163 expression was correlated with ASFV susceptibility. However, in this study the expression of porcine CD163 on cell lines did not increase the infection rate of these cells indicating other factors are likely to be important in determining susceptibility to infection. On adherent porcine bone marrow (pBM) cells the expression of CD45 was strongly correlated with infection. CD163 and CD203a expression correlated at intermediate levels with infection, indicating cells expressing these markers could become infected but were not preferentially infected by the virus. Most of the cells expressing MHCII were infected, indicating that they may be preferentially infected although expression of MHCII was not essential for infection and a large percentage of the infected cells were MHCII negative. CD16 showed a marked decrease in expression following infection and significantly lower levels of infected cells were shown to express CD16. Altogether these results suggest CD163 may be involved in ASFV infection but it may not be essential; the results also highlight the importance of other cell markers which requiring further investigation.
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影响因子：
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