Controlled induction of distributed microdeformation in wounded tissue via a microchamber array dressing.
Controlled induction of distributed microdeformation in wounded tissue via a microchamber array dressing.
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DOI：
10.1002/jbm.a.32840
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发表时间：
2010-11
影响因子：
4.9
通讯作者：
Orgill, Dennis P.
中科院分区：
文献类型：
--
作者：
Kane, Bartholomew J.;Younan, George;Helm, Douglas;Dastouri, Pouya;Prentice-Mott, Harrison;Irimia, Daniel;Chan, Rodney K.;Toner, Mehmet;Orgill, Dennis P.
关键词：
已知机械刺激在确定活细胞和组织的结构和功能中起重要作用。最近的研究强调了机械信号在哺乳动物皮肤伤口愈合中的作用。然而，受伤组织的机械刺激和随后的细胞反应之间的生物学联系尚未完全确定。研究人员研究这种联系的能力部分受到缺乏能够对受伤组织施加受控机械刺激的仪器的限制。本文概述的研究检验了以下假设：使用微制造敷料可以控制诱导伤口组织变形的幅度，该敷料由硅橡胶贴片中呈现的开放面、六边形微室阵列组成。通过将敷料连接到单个真空源，下面的受伤组织被吸入每个微室中，从而诱导组织变形。对于这些研究，将敷料应用于全层鼠皮肤伤口，200 mmHg真空12小时。研究表明，敷料能够诱导伤口组织变形，数值范围为11%-29%。通过统计分析，所引起的变形的大小被示出为微室高度和宽度的函数。这些结果表明，敷料能够控制下层组织的变形量。通过允许施加不同强度的机械刺激，这种敷料将能够在皮肤伤口愈合中进行复杂的机械生物学研究。
Mechanical stimuli are known to play an important role in determining the structure and function of living cells and tissues. Recent studies have highlighted the role of mechanical signals in mammalian dermal wound healing. However, the biological link between mechanical stimulation of wounded tissue and the subsequent cellular response has not been fully determined. The capacity for researchers to study this link is partially limited by the lack of instrumentation capable of applying controlled mechanical stimuli to wounded tissue. The studies outlined here tested the hypothesis that it was possible to control the magnitude of induced wound tissue deformation using a microfabricated dressing, comprised of an array of open-faced, hexagonally-shaped microchambers rendered in a patch of silicone rubber. By connecting the dressing to a single vacuum source, the underlying wounded tissue was drawn up into each of the microchambers, thereby inducing tissue deformation. For these studies, the dressings were applied to full thickness murine dermal wounds with 200 mmHg vacuum for 12 hours. The studies demonstrated that the dressing was capable of inducing wound tissue deformation with values ranging from 11 to 29 %. Through statistical analysis, the magnitude of the induced deformation was shown to be a function of both microchamber height and width. These results demonstrated that the dressing was capable of controlling the amount of deformation imparted in the underlying tissue. By allowing the application of mechanical stimulation with varying intensity, such a dressing will enable the performance of sophisticated mechanobiology studies in dermal wound healing.
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