Effects of Electroacupuncture on the Daily Rhythmicity of Intestinal Movement and Circadian Rhythmicity of Colonic Per2 Expression in Rats with Spinal Cord Injury.
Effects of Electroacupuncture on the Daily Rhythmicity of Intestinal Movement and Circadian Rhythmicity of Colonic Per2 Expression in Rats with Spinal Cord Injury.
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DOI：
10.1155/2016/9860281
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发表时间：
2016
影响因子：
--
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Cheng J;Wang X;Guo J;Yang Y;Zhang W;Xie B;Zhu Z;Lu Y;Zhu Y
背景资料。脊髓损伤(SCI)会导致肠功能障碍。电针(EA)可改善肠功能。目标。观察电针对脊髓损伤大鼠肠道运动的昼夜节律性和结肠PER2表达的昼夜节律性的影响。方法：研究方法。将大鼠随机分为假手术组、脊髓损伤组和脊髓损伤+电针组。脊髓损伤后于白天(11：00~11：30)行双侧足三里穴电针治疗14天。评估肠道转运和每日肠道运动的节律性。实时定量RT-PCR检测结肠PER2表达的昼夜节律性。结果。电针可缩短脊髓损伤大鼠24 h内大便排出时间，增加大便干重。每日肠道运动节律性不受脊髓损伤的影响。脊髓损伤组和假手术组大鼠结肠PER2表达于20：00达高峰，8：00达最低点。脊髓损伤+电针组大鼠结肠PER2表达在12：00和20：00达到高峰，8：00达到最低点。结论。脊髓损伤不改变大鼠结肠PER2表达的昼夜节律性，并保留每日肠道运动的节律性。电针改变脊髓损伤大鼠每日肠道运动的节律性和结肠PER2表达的昼夜节律性，使电针治疗后不久PER2的表达增加。
Background. Spinal cord injury (SCI) leads to bowel dysfunction. Electroacupuncture (EA) may improve bowel function. Objective. To assess EA on daily rhythmicity of intestinal movement and circadian rhythmicity of colonic Per2 expression in rats with SCI. Methods. Rats were randomized to the sham, SCI, and SCI+EA groups. EA was performed at bilateral Zusanli point (ST36) during daytime (11:00–11:30) for 14 days following SCI. Intestinal transit and daily rhythmicity of intestinal movement were assessed. Circadian rhythmicity of colonic Per2 expression was assessed by real-time RT-PCR. Results. EA shortened the stool efflux time and increased the dry fecal weight within 24 h in SCI rats. Daily rhythmicity of intestinal movements was unaffected by SCI. The expression of colonic Per2 peaked at 20:00 and the nadir was observed at 8:00 in the SCI and sham groups. In the SCI+EA group, colonic Per2 expression peaked at 12:00 and 20:00, and the nadir was observed at 8:00. Conclusion. SCI did not change the circadian rhythmicity of colonic Per2 expression in rats, and daily intestinal movement rhythmicity was retained. EA changed the daily rhythmicity of intestinal movement and the circadian rhythmicity of colonic Per2 expression in rats with SCI, increasing Per2 expression shortly after EA treatment.
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影响因子：
3.5
作者：
Liu, Chin-Wei;Huang, Chun-Chiang;Huang, Mao-Hsiung
通讯作者：
Huang, Mao-Hsiung
影响因子：
13.9
作者：
Mohawk JA;Green CB;Takahashi JS
通讯作者：
Takahashi JS
影响因子：
4.2
作者：
Scheff, SW;Rabchevsky, AG;Lumpp, JE
通讯作者：
Lumpp, JE
影响因子：
--
作者：
Hoogerwerf, Willemijntje A
通讯作者：
Hoogerwerf, Willemijntje A
影响因子：
4.3
作者：
Bostwick, Jonathon;Nguyen, Diane;Hoogerwerf, Willemijntje A.
通讯作者：
Hoogerwerf, Willemijntje A.