Nanoscale compositional mapping of cells, tissues, and polymers with ringing mode of atomic force microscopy.
Nanoscale compositional mapping of cells, tissues, and polymers with ringing mode of atomic force microscopy.
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DOI：
10.1038/s41598-017-12032-z
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发表时间：
2017-09-19
影响因子：
4.6
通讯作者：
Sokolov I
中科院分区：
文献类型：
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作者：
Dokukin ME;Sokolov I
最近开发的亚共振攻丝模式（如数字脉冲、峰值力攻丝、Hybrid等）原子力显微镜（AFM）的使用允许对（生物）材料和生物细胞的成分对比度成像到纳米级。在这里，我们报告了这些模式的一个强大的扩展，“振铃”模式，它的数量增加了一倍以上的非平凡的物理通道，可以收集一个定期的子共振轻敲。它可以同时记录五个新的额外的组成参数有关的粘合剂和粘弹性的样品表面：恢复（平均）的粘附力，粘附高度，拉脱颈高度，脱离距离，和脱离能量损失。与现有的亚共振轻敲模式相比，振铃模式可以快20倍，并且显示更少的伪影。振铃模式是基于AFM探针从样品表面分离后AFM悬臂的振铃信号的分析（该信号目前被视为噪声，并且通常在现有模式中被滤除）。我们证明，这种新模式允许记录固定的人类上皮细胞，角质细胞皮肤薄片和用于生物植入物的聚合物的强大和独特的信息。
Recently developed sub-resonance tapping modes (such as Digital Pulse, Peak Force Tapping, HybriD, etc.) of atomic force microscopy (AFM) allow imaging of compositional contrast of (bio)materials and biological cells down to the nanoscale. Here we report on a powerful extension of those modes, “ringing” mode, which more than doubles the number of non-trivial physical channels that can be collected with a regular sub-resonance tapping. It can simultaneously record five new additional compositional parameters related to adhesive and viscoelastic properties of the sample surface: the restored (averaged) adhesion, adhesion height, pull-off neck height, detachment distance, and detachment energy losses. Ringing mode can be up to 20 times faster and showing fewer artifacts compared to the existing sub-resonance tapping modes. Ringing mode is based on an analysis of ringing signal of the AFM cantilever after detaching the AFM probe from the sample surface (this signal is currently treated as noise, and typically filtered out in the existing modes). We demonstrate that this new mode allows recording robust and unique information on fixed human epithelial cells, corneocyte skin flakes, and polymers used for bioimplants.
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影响因子：
2.2
作者：
Guz, N. V.;Gaikwad, R. M.;Sokolov, I.
通讯作者：
Sokolov, I.
影响因子：
6.7
作者：
Stan, Gheorghe;Solares, Santiago D.;Su, Chanmin
通讯作者：
Su, Chanmin
影响因子：
3.5
作者：
Berdyyeva, TK;Woodworth, CD;Sokolov, I
通讯作者：
Sokolov, I
影响因子：
56.9
作者：
Rief, M;Oesterhelt, F;Gaub, HE
通讯作者：
Gaub, HE
影响因子：
38.3
作者：
通讯作者：
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