Assessing the microbiota of the snail intermediate host of trematodes, Galba truncatula.
Assessing the microbiota of the snail intermediate host of trematodes, Galba truncatula.
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评估蜗牛中间寄主的微生物，Galba Truncatula。
DOI：
10.1186/s13071-024-06118-7
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发表时间：
2024-01-23
影响因子：
3.2
通讯作者：
Gobert, Geoffrey N.
中科院分区：
文献类型：
--
作者：
Mccann, Peter;Mcfarland, Christopher;Megaw, Julianne;Siu-Ting, Karen;Cantacessi, Cinzia;Rinaldi, Gabriel;Gobert, Geoffrey N.
众所周知，微生物群在健康和疾病中发挥着关键作用，包括宿主对寄生虫感染的易感性。元宝螺是多种吸虫的中间宿主，包括肝脏吸虫和瘤胃吸虫、肝片吸虫和多布尼氏吸虫。此前曾对蜗牛-寄生虫系统进行过调查。然而，钉螺相关微生物区系与钉螺内吸虫发育阶段之间的具体相互作用仍有待探索。从北爱尔兰的养殖场采集元宝螺，用聚合酶链式反应诊断吸虫感染。随后，对细菌16S核糖体DNA V3-V4高变区进行了高通量测序分析，以确定未感染和感染钉螺的微生物区系。我们首先展示了蜗牛栖息的微生物区系，这种微生物区系与其所处的环境截然不同。感染钉螺的微生物区系与未感染钉螺的微生物区系有显著差异。特别是，支原体和甲基洛格氏菌属细菌在感染的钉螺中显著丰富，而鞘氨醇单胞菌和诺卡氏菌属主要与未感染的钉螺有关。这些发现为未来研究细菌在宿主-寄生虫关系中的功能作用铺平了道路。网上版载有补充材料，可在10.1186/s13071-024-06118-7查阅。
The microbiome is known to play key roles in health and disease, including host susceptibility to parasite infections. The freshwater snail Galba truncatula is the intermediate host for many trematode species, including the liver and rumen flukes Fasciola hepatica and Calicophoron daubneyi, respectively. The snail-parasite system has previously been investigated. However, the specific interaction between the snail-associated microbiota and intra-snail developmental stages of trematodes has yet to be explored. Galba truncatula snails were collected from farms in Northern Ireland and trematode infection was diagnosed using PCR. High-throughput sequencing analysis of the bacterial 16S ribosomal DNA V3-V4 hypervariable regions was subsequently applied to characterise the microbiota of both uninfected and infected snails. We first showed that the snail harboured microbiota that was distinct for its environment. The microbiota of infected snails was found to differ significantly from that of uninfected snails. In particular, the bacterial genera Mycoplasma and Methylotenera were significantly more abundant in infected snails, while genera Sphingomonas and Nocardioides were predominantly associated with uninfected snails. These findings pave the way to future studies on the functional roles of bacteria in host-parasite relationships. The online version contains supplementary material available at 10.1186/s13071-024-06118-7.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
3.4
作者：
Bennett, R
通讯作者：
Bennett, R
影响因子：
3.7
作者：
Braquart-Varnier C;Lachat M;Herbinière J;Johnson M;Caubet Y;Bouchon D;Sicard M
通讯作者：
Sicard M
影响因子：
6.4
作者：
Dheilly NM;Bolnick D;Bordenstein S;Brindley PJ;Figuères C;Holmes EC;Martínez Martínez J;Phillips AJ;Poulin R;Rosario K
通讯作者：
Rosario K
影响因子：
15.5
作者：
Davis, Nicole M;Proctor, Diana M;Callahan, Benjamin J
通讯作者：
Callahan, Benjamin J