Wolbachia mediate variation of host immunocompetence.
Wolbachia mediate variation of host immunocompetence.
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DOI：
10.1371/journal.pone.0003286
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发表时间：
2008-09-26
期刊：
影响因子：
3.7
通讯作者：
Sicard M
中科院分区：
文献类型：
--
作者：
Braquart-Varnier C;Lachat M;Herbinière J;Johnson M;Caubet Y;Bouchon D;Sicard M
几十年来，人们一直认为内共生菌在宿主体内是沉默的，尤其是在免疫系统方面，但最近的研究揭示了相反的情况。本文研究了节肢动物中最常见的内共生菌沃尔巴克氏体对宿主免疫能力的影响。为此，我们选择了a . vulgus -Wolbachia共生作为模型系统，因为它可以比较两种Wolbachia菌株（wVulC和wVulM）对同一种群宿主的影响。此外，在负责体液和细胞免疫反应的血细胞中直接观察到沃尔巴克氏体是唯一的宿主物种。我们从非共生、感染wVulC或感染wVulM的同一种群中取样妊娠雌性。对这些雌性的后代进行了测试，发现携带wVulC的个体表现出：(1)血细胞密度较低，(2)血淋巴败血症更严重，(3)与携带wVulM或非共生的个体相比，寿命较短。因此，这个种群中的个体似乎更容易感染wVulC而不是wVulM。两种沃尔巴克氏菌株的共生滴度和在血细胞中的位置没有差异，这表明这两个参数不是在普通单胞杆菌中观察到的扩展表型差异的原因。两种沃尔巴克氏体菌株感染同一种群的普通单胞虫后，其宿主的免疫能力和存活率发生了变化。这种变化应该高度影响这个宿主-共生体系统的动态。根据以往的群体遗传学研究，我们提出wVulM是一种通过长期适应当地普通a .基因型而减弱其毒力的本地菌株，而wVulC是一种广泛存在的入侵菌株，不具有本地适应性。
After decades during which endosymbionts were considered as silent in their hosts, in particular concerning the immune system, recent studies have revealed the contrary. In the present paper, we addressed the effect of Wolbachia, the most prevalent endosymbiont in arthropods, on host immunocompetence. To this end, we chose the A. vulgare-Wolbachia symbiosis as a model system because it leads to compare consequences of two Wolbachia strains (wVulC and wVulM) on hosts from the same population. Moreover, A. vulgare is the only host-species in which Wolbachia have been directly observed within haemocytes which are responsible for both humoral and cellular immune responses. We sampled gravid females from the same population that were either asymbiotic, infected with wVulC, or infected with wVulM. The offspring from these females were tested and it was revealed that individuals harbouring wVulC exhibited: (i) lower haemocyte densities, (ii) more intense septicaemia in their haemolymph and (iii) a reduced lifespan as compared to individuals habouring wVulM or asymbiotic ones. Therefore, individuals in this population of A. vulgare appeared to suffer more from wVulC than from wVulM. Symbiotic titer and location in the haemocytes did not differ for the two Wolbachia strains showing that these two parameters were not responsible for differences observed in their extended phenotypes in A. vulgare. The two Wolbachia strains infecting A. vulgare in the same population induced variation in immunocompetence and survival of their hosts. Such variation should highly influence the dynamics of this host-symbiont system. We propose in accordance with previous population genetic works, that wVulM is a local strain that has attenuated its virulence through a long term adaptation process towards local A. vulgare genotypes whereas wVulC, which is a widespread and invasive strain, is not locally adapted.
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影响因子：
1.9
作者：
De Barro, PJ;Hart, PJ
通讯作者：
Hart, PJ
DOI：
10.1098/rspb.1998.0402
发表时间：
1998-06-22
影响因子：
4.7
作者：
Bouchon, D;Rigaud, T;Juchault, P
通讯作者：
Juchault, P
DOI：
10.1098/rspb.2005.3383
发表时间：
2006-04-07
影响因子：
4.7
作者：
Fytrou, A;Schofield, PG;Hubbard, SF
通讯作者：
Hubbard, SF
DOI：
10.1073/pnas.101304298
发表时间：
2001-05-22
影响因子：
11.1
作者：
Dedeine, F;Vavre, F;Boulétreau, M
通讯作者：
Boulétreau, M
影响因子：
3.7
作者：
Brennan LJ;Keddie BA;Braig HR;Harris HL
通讯作者：
Harris HL