A reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice.
A reproducible protocol for neonatal ischemic injury and cardiac regeneration in neonatal mice.
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DOI：
10.1007/s00395-016-0580-3
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发表时间：
2016-11
影响因子：
9.5
通讯作者：
Penninger JM
中科院分区：
文献类型：
--
作者：
Haubner BJ;Schuetz T;Penninger JM
心脏再生是心血管研究的主要目标之一。小鼠新生儿心尖切除和左前降支动脉（LAD）结扎模型引入了新的体内哺乳动物试验，以研究心脏再生。然而，最近的报告和社论讨论和批判性地质疑小鼠新生儿心肌梗死方法的价值和技术可重复性，使得开发和使用可重复的模型系统至关重要。我们建立了一个小鼠新生儿心肌梗死模型，通过视觉确认结扎前降支，使用显微外科手术。TdT介导的dUTP缺口末端标记（TUNEL）证明了新生小鼠和7日龄小鼠心尖和前壁的一个确定区域可重复的大面积心肌梗死。出生后第7天结扎的心脏显示慢性损伤，而出生后立即结扎的心脏样本在长期随访后总是显示完全的结构再生。在迄今为止测试的所有小鼠品系（C57 BL/6 J、ICR和混合背景C57 BL/6 JxSv 129）中均观察到心脏再生。我们提出了一个详细的体内方案，以研究复杂的机制，完全心脏修复缺血性心脏损伤。新生儿LAD结扎手术是可行的，结扎后24小时可重复心肌梗死，长期随访后未检测到结构性心肌缺损。我们鼓励心血管社区使用我们的协议和教学视频来回答心脏再生领域的关键科学问题。本文的在线版本（doi：10.1007/s 00395 -016-0580-3）包含补充材料，可供授权用户使用。
Cardiac regeneration is one of the prime visions in cardiovascular research. The mouse neonatal apical resection and left anterior descending artery (LAD) ligation model introduced novel in vivo mammalian assays to study cardiac regeneration. However, recent reports and editorials discussed and critically questioned the value and technical reproducibility of the mouse neonatal myocardial infarction approach, making it paramount to develop and use a reproducible model system. We established a mouse neonatal myocardial infarction model by visually confirmed ligation of the LAD using microsurgery. TdT-mediated dUTP nick-end labeling (TUNEL) proved reproducible massive myocardial infarctions in a defined region of the apex and anterior wall of neonatal and 7-day-old mice. Whereas hearts ligated on postnatal day 7 displayed chronic injury, cardiac samples ligated immediately after birth always showed complete structural regeneration after long-term follow-up. Cardiac regeneration was observed in all mouse stains (C57BL/6J, ICR, and mixed background C57BL/6JxSv129) tested so far. We present a detailed in vivo protocol to study complex mechanisms of complete cardiac repair following ischemic cardiac damage. Neonatal LAD ligation surgery is feasible, and results in reproducible myocardial infarctions 24 h after ligation, and no structural myocardial defects are detectable following long-term follow-up. We encourage the cardiovascular community to use our protocol and teaching video to answer key scientific questions in the field of cardiac regeneration. The online version of this article (doi:10.1007/s00395-016-0580-3) contains supplementary material, which is available to authorized users.
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影响因子：
2.7
作者：
Darehzereshki, Ali;Rubin, Nicole;Gamba, Laurent;Kim, Jieun;Fraser, James;Huang, Ying;Billings, Joshua;Mohammadzadeh, Robabeh;Wood, John;Warburton, David;Kaartinen, Vesa;Lien, Ching-Ling
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Lien, Ching-Ling
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56.9
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通讯作者：
Keating, MT
DOI：
10.1073/pnas.1208863110
发表时间：
2013-01-02
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通讯作者：
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影响因子：
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Moran AE;Forouzanfar MH;Roth GA;Mensah GA;Ezzati M;Murray CJ;Naghavi M
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