GmNFYA13 Improves Salt and Drought Tolerance in Transgenic Soybean Plants.
GmNFYA13 Improves Salt and Drought Tolerance in Transgenic Soybean Plants.
复制标题
GmNFYA13 提高转基因大豆植物的耐盐性和耐旱性
DOI：
10.3389/fpls.2020.587244
复制
发表时间：
2020
影响因子：
5.6
通讯作者：
Ma YZ
中科院分区：
文献类型：
--
作者：
Ma XJ;Fu JD;Tang YM;Yu TF;Yin ZG;Chen J;Zhou YB;Chen M;Xu ZS;Ma YZ
核因子-YA转录因子在调节对作物产量构成严重威胁的非生物胁迫的耐受性方面发挥作用。在本研究中，大豆中的一个NF-YA基因GmNFYA13被盐、干旱、ABA和H_2O_2强烈诱导，并被ABA合成抑制剂钨酸盐抑制。GmNFYA13基因在苗期和开花期的不同组织中均有表达，其中根中的表达水平最高。GmNFYA13是一种具有自激活活性的核定位蛋白。与WT植株相比，高表达GmNFYA13基因的转基因植株表现出对ABA的超敏反应，并增强了对盐和干旱胁迫的耐受性。此外，过表达GmNFYA13导致了OE大豆植株更强的耐盐性和抗旱性，而抑制GmNFYA13则产生了相反的结果。此外，GmNFYA13还可以与GmSALT3、GmMYB84、GmNCED3和GmRbohB启动子结合，调节它们在体内的表达丰度。本研究结果表明，GmNFYA13提高了大豆植株的耐盐性和抗旱性。
NF-YA transcription factors function in modulating tolerance to abiotic stresses that are serious threats to crop yields. In this study, GmNFYA13, an NF-YA gene in soybean, was strongly induced by salt, drought, ABA, and H2O2, and suppressed by tungstate, an ABA synthesis inhibitor. The GmNFYA13 transcripts were detected in different tissues in seedling and flowering stages, and the expression levels in roots were highest. GmNFYA13 is a nuclear localization protein with self-activating activity. Transgenic Arabidopsis plants overexpressing GmNFYA13 with higher transcript levels of stress-related genes showed ABA hypersensitivity and enhanced tolerance to salt and drought stresses compared with WT plants. Moreover, overexpression of GmNFYA13 resulted in higher salt and drought tolerance in OE soybean plants, while suppressing it produced the opposite results. In addition, GmNFYA13 could bind to the promoters of GmSALT3, GmMYB84, GmNCED3, and GmRbohB to regulate their expression abundance in vivo. The data in this study suggested that GmNFYA13 enhanced salt and drought tolerance in soybean plants.
登录
查看更多内容
影响因子：
4.3
作者：
Imahori, Yoshihiro;Bai, Jinhe;Baldwin, Elizabeth
通讯作者：
Baldwin, Elizabeth
影响因子：
5.6
作者：
Golldack D;Li C;Mohan H;Probst N
通讯作者：
Probst N
影响因子：
11.4
作者：
Hao, Gang-Ping;Zhang, Xiu-Hai;Huang, Cong-Lin
通讯作者：
Huang, Cong-Lin
影响因子：
4.3
作者：
Bihmidine, Saadia;Lin, Jiusheng;Clemente, Tom E.
通讯作者：
Clemente, Tom E.
影响因子：
5.2
作者：
Lee, Dong-Keun;Kim, Hyung Il;Kim, Ju-Kon
通讯作者：
Kim, Ju-Kon