Big Grain3, encoding a purine permease, regulates grain size via modulating cytokinin transport in rice.
Big Grain3, encoding a purine permease, regulates grain size via modulating cytokinin transport in rice.
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Big Grain3 编码嘌呤渗透酶，通过调节水稻中的细胞分裂素转运来调节颗粒大小。
DOI：
10.1111/jipb.12727
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发表时间：
2018-12
影响因子：
11.4
通讯作者：
Chu Chengcai
中科院分区：
文献类型：
--
作者：
Xiao Yunhua;Liu Dapu;Zhang Guoxia;Gao Shaopei;Liu Linchuan;Xu Fan;Che Ronghui;Wang Yiqin;Tong Hongning;Chu Chengcai
籽粒大小是影响籽粒产量的重要农艺性状，但其分子机制仍有待阐明。在这里，我们分离出一个显性突变体，大颗粒3（bg 3-D），表现出显着增加的颗粒大小所引起的嘌呤渗透酶基因，OsPUP 4的激活。BG 3/OsPUP 4主要在血管组织中表达，并且被外源细胞分裂素应用特异性抑制。激素分析显示，不同的细胞分裂素形式的分布，在根和芽的bg 3-D突变体，被改变。定量逆转录-PCR（qRT-PCR）分析表明，水稻细胞分裂素A型反应调节因子（OsRR）基因在bg 3-D突变体的根中的表达增强。这些结果表明，OsPUP 4可能有助于细胞分裂素的长距离运输，通过加强细胞分裂素加载到维管束细胞。此外，过表达OsPUP 4的最接近同源物OsPUP 7的植物也表现出与bg 3-D突变体相似的表型。有趣的是，亚细胞定位表明OsPUP 4定位于质膜上，而OsPUP 7定位于内质网。基于这些发现，我们提出OsPUP 4和OsPUP 7在线性途径中起作用，以指导细胞分裂素细胞到细胞的运输，影响其长距离运动和局部分配。
Grain size is an important agronomic trait affecting grain yield, but the underlying molecular mechanisms remain to be elucidated. Here, we isolated a dominant mutant, big grain3 (bg3-D), which exhibits a remarkable increase of grain size caused by activation of the PURINE PERMEASE gene, OsPUP4. BG3/OsPUP4 is predominantly expressed in vascular tissues and is specifically suppressed by exogenous cytokinin application. Hormone profiling revealed that the distribution of different cytokinin forms, in roots and shoots of the bg3-D mutant, is altered. Quantitative reverse transcription-PCR (qRT-PCR) analysis indicated that expression of rice cytokinin type-A RESPONSE REGULATOR (OsRR) genes is enhanced in the roots of the bg3-D mutant. These results suggest that OsPUP4 might contribute to the long-distance transport of cytokinin, by reinforcing cytokinin loading into vascular bundle cells. Furthermore, plants overexpressing OsPUP7, the closest homolog of OsPUP4, also exhibited a similar phenotype to the bg3-D mutant. Interestingly, subcellular localization demonstrated that OsPUP4 was localized on the plasma membrane, whereas OsPUP7 was localized to the endoplasmic reticulum. Based on these findings, we propose that OsPUP4 and OsPUP7 function in a linear pathway to direct cytokinin cell-to-cell transport, affecting both its long-distance movement and local allocation.
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影响因子：
7.4
作者：
M. Mok;Ruth C. Martin;P. Dobrev;R. Vaňková;P. Shing Ho;K. Yonekura-Sakakibara;H. Sakakibara;D. M
通讯作者：
M. Mok;Ruth C. Martin;P. Dobrev;R. Vaňková;P. Shing Ho;K. Yonekura-Sakakibara;H. Sakakibara;D. M
影响因子：
16.6
作者：
Kewei Zhang;O. Novák;Zhaoyang Wei;Mingyue Gou;Xuebin Zhang;Yong Yu;Huijun Yang;Yuanheng Cai;M. Strnad;Chang‐Jun Liu
通讯作者：
Kewei Zhang;O. Novák;Zhaoyang Wei;Mingyue Gou;Xuebin Zhang;Yong Yu;Huijun Yang;Yuanheng Cai;M. Strnad;Chang‐Jun Liu
影响因子：
5.9
作者：
Ma, Yimian;Liu, Luo;Chu, Chengcai
通讯作者：
Chu, Chengcai
影响因子：
7.4
作者：
Hirose, N;Makita, N;Sakakibara, H
通讯作者：
Sakakibara, H
影响因子：
56.9
作者：
Zurcher, Evelyne;Liu, Jingchun;Muller, Bruno
通讯作者：
Muller, Bruno