Chemically enhanced lipid production from microalgae under low sub-optimal temperature
Chemically enhanced lipid production from microalgae under low sub-optimal temperature
复制标题
在次优低温下化学增强微藻的脂质生产
DOI：
10.1016/j.algal.2016.02.022
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发表时间：
2016-06
影响因子：
5.1
通讯作者：
Chen Yi-Feng
中科院分区：
文献类型：
--
作者：
Wang Yuancong;He Bing;Sun Zhilan;Chen Yi-Feng
低的次优温度是显着影响微藻生长和脂质产生的主要环境因素之一。然而，到目前为止，几乎没有研究已经完成，以估计和减少低次优温度（通常为15 °C-20 °C）对藻类脂质生产力的影响，藻类脂质用作生物柴油的原料。我们通过在低的次最适温度（18 °C）下培养小球藻（Chlorella sorokiniana）来解决这个问题（最适温度为26 °C，其用作对照）。与26 °C相比，藻类生长在18 °C下受到抑制，导致总体脂质生产率降低;然而，降低的温度增加了藻类细胞的总脂质含量。重要的是，与26 °C的对照处理相比，通过施用500 mg/L的外源甘氨酸甜菜碱改善了低亚适温度下降低的脂质生产率。定量PCR分析表明，外源甜菜碱增强了Rubisco基因的表达，Rubisco是参与光合作用固碳的关键酶之一。在18 °C和26 °C下，甘氨酸甜菜碱处理均上调了参与卡尔文循环的其他关键基因的表达。此外，甘氨酸甜菜碱处理对藻类脂肪酸谱的影响很小，因此它不影响十六烷值，十六烷值是生物柴油质量的关键参数。总而言之，我们的证据支持在低的次最佳温度下化学增强微藻的脂质产量，其脂质产量与最佳温度下的脂质产量相当。甘氨酸甜菜碱的新应用可以通过将种植的地理范围扩展到气候较冷的地区来加强藻类生物柴油工业。
Low sub-optimal temperatures are one of the major environmental factors that significantly affects growth and lipid production of microalgae. However, until now little research has been done to estimate and reduce the effects of low sub-optimal temperatures (typically 15 °C–20 °C) on productivity of algal lipids, which serve as feedstock for biodiesel. We addressed this issue by growing the microalga Chlorella sorokiniana under a low sub-optimal temperature (18 °C) (the optimal temperature was 26 °C, which was used as a control). Algal growth was inhibited at 18 °C compared to 26 °C, resulting in overall decreased lipid productivity; however, decreased temperature increased the total lipid content of the algal cell. Importantly, decreased lipid productivity under the low sub-optimal temperature was improved by an application of exogenous glycine betaine at 500 mg/L compared to the control treatment of 26 °C. Quantitative PCR analysis showed that exogenous glycine betaine enhanced expression of genes encoding Rubisco, one of the key enzymes involved in carbon fixation through photosynthesis. The expression of other key genes involved in the Calvin cycle was up-regulated by treatment with glycine betaine at both 18 °C and 26 °C. In addition, glycine betaine treatment had little influence on algal fatty acid profiling, hence it did not affect the cetane number that is a key parameter of biodiesel quality. Taken together, our evidence supports a chemically enhanced lipid production of microalgae under low sub-optimal temperatures whose lipid output was comparable to those at optimal temperatures. The novel application of glycine betaine could strengthen the algal biodiesel industry by extending the geographical range for cultivation into areas with cooler climates.
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影响因子：
6.3
作者：
Parsaeimehr A;Sun Z;Dou X;Chen YF
通讯作者：
Chen YF
影响因子：
13.8
作者：
Sun, Zhilan;Chen, Yi-Feng;Du, Jianchang
通讯作者：
Du, Jianchang
DOI：
10.1016/b978-0-12-385099-7.00018-8
发表时间：
2011
期刊：
--
影响因子：
--
作者：
Chen Yi-feng;Qingyu Wu
通讯作者：
Chen Yi-feng;Qingyu Wu
DOI：
10.1016/j.cep.2009.03.006
发表时间：
2009-06-01
影响因子：
4.3
作者：
Converti, Attilio;Casazza, Alessandro A.;Del Borghi, Marco
通讯作者：
Del Borghi, Marco
影响因子：
7.3
作者：
KISHITANI, S;WATANABE, K;TAKABE, T
通讯作者：
TAKABE, T