The NMDA-NR1 receptor subunit and the mu-opioid receptor are expressed in somatodendritic compartments of central nucleus of the amygdala neurons projecting to the bed nucleus of the stria terminalis.
The NMDA-NR1 receptor subunit and the mu-opioid receptor are expressed in somatodendritic compartments of central nucleus of the amygdala neurons projecting to the bed nucleus of the stria terminalis.
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DOI：
10.1016/j.expneurol.2011.12.034
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发表时间：
2012-03
影响因子：
5.3
通讯作者：
Glass, Michael J.
中科院分区：
文献类型：
--
作者：
Beckerman, Marc A.;Glass, Michael J.
杏仁核中央核（CeA）和终纹床核（BNST）之间的通路正在成为应激相关情感过程的重要介质。证据还表明，暴露于滥用药物，如阿片类药物，与CeA和BNST中的NMDA型谷氨酸受体依赖性可塑性相关。然而，很少有证据表明，NMDA受体表达在CeA神经元投射到BNST，或阿片类药物诱导的BNST神经激活所需的。采用免疫电镜、束示踪和条件基因缺失技术研究了CeA-BNST通路中NMDA受体和μ阿片受体（μOR）的突触结构。双标电镜显示，CeA-BNST投射神经元表达NMDA-NR 1受体亚单位（NR 1）或μOR。三重标记还发现NR 1和μOR在部分CeA-BNST投射神经元中共表达。尽管NR 1和μOR共定位于CeA神经元的体-树突隔室，但在BNST的轴突终末很少表达。删除CeA神经元中的NR 1基因导致吗啡诱导的腹侧BNST中Fos蛋白标记减少。总之，NR 1和μOR在CeA神经元的体树突部位共表达，包括投射到BNST的部位。此外，NR 1基因在CeA神经元中的表达是吗啡诱导的BNST神经激活所必需的。因此，突触后NMDA受体和μORs定位于共同调节CeA投射神经元到BNST，这可能为阿片成瘾行为中关键参与的应激诱导的情绪过程提供突触底物。
The pathway between the central nucleus of the amygdala (CeA) and the bed nucleus of the stria terminalis (BNST) is emerging as a critical mediator of stress-related affective processes. Evidence also indicates that exposure to drugs of abuse, like opioids, is associated with NMDA-type glutamate receptor-dependent plasticity in the CeA and BNST. However, there is little evidence that NMDA receptors are expressed in CeA neurons projecting to the BNST, or are required for opioid-induced BNST neural activation. Immunoelectron microscopy, tract tracing, and conditional gene deletion technology were used to investigate the synaptic organization of the NMDA receptor and the mu-opioid receptor (μOR) in the CeA-BNST pathway. By dual labeling electron microscopy, numerous CeA-BNST projection neurons expressed the NMDA-NR1 receptor subunit (NR1) or μOR. By triple labeling, it was also found that NR1 and μOR were co-expressed in some CeA-BNST projection neurons. Despite being colocalized in somato-dendritic compartments of CeA neurons, NR1 and μOR were rarely expressed in their axonal terminations in the BNST. Deleting the NR1 gene in CeA neurons resulted in a reduction of morphine-induced Fos protein labeling in the ventral BNST. In summary, NR1 and μOR are coexpressed in somatodendritic sites of CeA neurons, including those projecting to the BNST. In addition, expression of the NR1 gene in CeA neurons is required for morphine-induced BNST neural activation. Thus, postsynaptic NMDA receptors and μORs are positioned for the co-modulation of CeA projection neurons to the BNST, which may provide a synaptic substrate for stress-induced emotional processes critically involved in opioid addictive behaviors.
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在大鼠和人类中持续的恐惧：恐惧与焦虑中的杏仁核的作用。
DOI：
10.1038/npp.2009.109
发表时间：
2010-01
期刊：
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.3
作者：
Glass, M. J.;Vanyo, L.;Quimson, L.;Pickel, V. M.
通讯作者：
Pickel, V. M.
影响因子：
2.3
作者：
Glass, MJ;Colago, EEO;Pickel, VM
通讯作者：
Pickel, VM
影响因子：
1.9
作者：
Andrzejewski, ME;Sadeghian, K;Kelley, AE
通讯作者：
Kelley, AE
影响因子：
3.3
作者：
Jaferi, A.;Pickel, V. M.
通讯作者：
Pickel, V. M.