Antimicrobial photodynamic therapy-a promising treatment for prosthetic joint infections.
Antimicrobial photodynamic therapy-a promising treatment for prosthetic joint infections.
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DOI：
10.1007/s10103-017-2394-4
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发表时间：
2018-04
影响因子：
2.1
通讯作者：
Coathup M
中科院分区：
文献类型：
--
作者：
Briggs T;Blunn G;Hislop S;Ramalhete R;Bagley C;McKenna D;Coathup M
假体周围关节感染（PJI）与高患者发病率和巨大的经济成本相关。本研究调查了光动力疗法（PDT）作为根除导致PJI的细菌的一种手段，使用波长为665 nm的激光和亚甲蓝（MB）作为光敏剂。研究了MB浓度对甲氧西林敏感金黄色葡萄球菌（MSSA）、耐甲氧西林金黄色葡萄球菌（MRSA）、表皮葡萄球菌、铜绿假单胞菌和鲍曼不动杆菌生长抑制的有效性。还研究了激光剂量的影响，并使用优化的PDT方法来研究其对微生物培养物内的物种的杀菌作用，以及在抛光钛和羟基磷灰石涂层钛盘上形成生物膜后的杀菌作用。结果表明，0.1 mM亚甲蓝（MB）的最低浓度下，葡萄球菌被根除。铜绿假单胞菌和A.增加MB浓度可提高杀菌效果。结果表明，随着激光剂量的增加，激光功率越大，杀灭细菌的能力越强，当激光功率≥ 35 J/cm 2，辐照度为35 mW/cm 2时，S.表皮。优化后的PDT方法对耐甲氧西林金黄色葡萄球菌（MRSA）和沙门氏菌（S.与单独MB、单独激光或对照（未处理）相比，当生物膜形成时，PDT处理对于在抛光盘表面上研究的所有种类的细菌具有比单独MB和单独激光显著更高的杀菌效果。与葡萄球菌相比，生长在生物膜中的铜绿假单胞菌对PDT的敏感性较低，HA涂层表面降低了PDT的有效性。这项研究表明，PDT可有效杀死导致PJI的细菌。
Periprosthetic joint infection (PJI) is associated with high patient morbidity and a large financial cost. This study investigated Photodynamic Therapy (PDT) as a means of eradicating bacteria that cause PJI, using a laser with a 665-nm wavelength and methylene blue (MB) as the photosensitizer. The effectiveness of MB concentration on the growth inhibition of methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Pseudomonas aeruginosa and Acinetobacter baumannii was investigated. The effect of laser dose was also investigated and the optimized PDT method was used to investigate its bactericidal effect on species within planktonic culture and following the formation of a biofilm on polished titanium and hydroxyapatite coated titanium discs. Results showed that Staphylococci were eradicated at the lowest concentration of 0.1 mM methylene blue (MB). With P. aeruginosa and A. baumannii, increasing the MB concentration improved the bactericidal effect. When the laser dose was increased, results showed that the higher the power of the laser the more bacteria were eradicated with a laser power ≥ 35 J/cm2 and an irradiance of 35 mW/cm2, eradicating all S. epidermidis. The optimized PDT method had a significant bactericidal effect against planktonic MRSA and S. epidermidis compared to MB alone, laser alone, or control (no treatment). When biofilms were formed, PDT treatment had a significantly higher bactericidal effect than MB alone and laser alone for all species of bacteria investigated on the polished disc surfaces. P. aeruginosa grown in a biofilm was shown to be less sensitive to PDT when compared to Staphylococci, and a HA-coated surface reduced the effectiveness of PDT. This study demonstrated that PDT is effective for killing bacteria that cause PJI.
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DOI：
10.1016/s0304-4165(00)00071-4
发表时间：
2000-07-03
影响因子：
3
作者：
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通讯作者：
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影响因子：
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DOI：
10.1016/j.jphotobiol.2011.10.001
发表时间：
2012-01-05
影响因子：
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作者：
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作者：
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影响因子：
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