Potential degradation of swainsonine by intracellular enzymes of Arthrobacter sp. HW08.
Potential degradation of swainsonine by intracellular enzymes of Arthrobacter sp. HW08.
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节杆菌胞内酶对苦马豆素的潜在降解。
DOI：
10.3390/toxins5112161
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发表时间：
2013-11-14
期刊：
影响因子：
4.2
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Wang Y;Li Y;Hu Y;Li J;Yang G;Kang D;Li H;Wang J
苦马豆素是由疯草产生的一种毒素，对畜牧业有害。利用节杆菌HW 08降解SW是一种很有前途的处理SW中毒的方法。然而，目前还不清楚节杆菌属HW 08中的亚细胞酶的哪一部分负责生物降解SW以及代谢产物是否无毒。本研究对节杆菌HW 08菌株的胞内和胞外酶进行了分离和活性测定。将代谢产物喂给小鼠，并研究了处理小鼠的生理和组织学特性。结果表明，只有节杆菌HW 08（IEHW 08）的胞内酶能有效降解SW。与SW治疗组相比，SW + IEHW 08治疗组小鼠（1）体重增加;（2）血小板数量增加，白色细胞数量减少;（3）血清肌酐、尿素氮、丙氨酸转氨酶和天冬氨酸转氨酶水平降低;（4）小脑、肝脏和肾脏空泡化细胞数量减少。所有这些数据表明，IEHW 08是潜在的安全的小鼠，同时保持的能力，降解SW。这项研究表明，IEHW 08作为一种添加剂在畜牧业，以保护动物免受SW中毒的可能应用。
Swainsonine (SW) is a toxin produced by locoweeds and harmful to the livestock industry. Degrading SW by Arthrobacter sp. HW08 was demonstrated as a promising way to deal with SW poisoning. However, it is unknown which part of the subcellular enzymes in Arthrobacter sp. HW08 is responsible for biodegrading SW and whether the metabolites are atoxic. In this study, intracellular and extracellular enzymes of Arthrobacter sp. HW08 were isolated and their enzyme activity was evaluated. The metabolites were fed to mice, and physiological and histological properties of the treated mice were investigated. The results showed that only intracellular enzyme of Arthrobacter sp. HW08 (IEHW08) could degrade SW efficiently. Compared with mice in SW treatment group, mice in SW + IEHW08 treatment group (1) increased their body weights; (2) showed higher number of platelets and lower number of white blood cells; (3) decreased the levels of creatinine, urea nitrogen, alanine transaminase and aspartate aminotransferase in serum; (4) reduced the number of vacuolated cells in cerebellum, liver and kidney. All these data demonstrate that IEHW08 was potentially safe for mice, while keeping the capacity of degrading SW. This study indicates a possible application of IEHW08 as an additive in the livestock industry to protect animals from SW poisoning.
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影响因子：
2.8
作者：
Yu, Yongtao;Zhao, Qingmei;Li, Qinfan
通讯作者：
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影响因子：
3.3
作者：
BACHMAN, SE;GALYEAN, ML;GRAHAM, JD
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影响因子：
1.5
作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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