Age-Dependent Changes in Total and Free Water Content of In Vivo Human Lenses Measured by Magnetic Resonance Imaging.
Age-Dependent Changes in Total and Free Water Content of In Vivo Human Lenses Measured by Magnetic Resonance Imaging.
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DOI：
10.1167/iovs.62.9.33
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发表时间：
2021-07-01
影响因子：
4.4
通讯作者：
Donaldson PJ
中科院分区：
文献类型：
--
作者：
Lie AL;Pan X;White TW;Vaghefi E;Donaldson PJ
目的：利用磁共振成像（MRI）技术测量人晶状体中总水和游离水的年龄依赖性变化。招募了64名年龄在18至86岁之间的健康成年人，配备了32通道头部接收器线圈，并放置在3特斯拉临床MR扫描仪中。使用具有双翻转角的容积内插屏气检查序列获得晶体透镜的扫描，使用使用涡轮闪光序列获得的B1图校正采集后的视野不均匀性。使用自定义编写的代码提取校正后的总水（ρ透镜）和游离水（T1）沿着透镜光轴的空间分布和含量。透镜总水分分布及含量随年龄的变化无显著性差异（均P > 0.05）。与总水相比，在所有年龄段的镜片中，游离水含量的梯度在周边相对于中心最高。然而，这种最初的抛物线自由水梯度逐渐发展出一个增强的中央高原，如剖面形状参数值（前部：0.067/y，P = 0.004;后部：0.050/y，P = 0.020）和中央自由水含量（1.932 ms/y，P = 0.022）随年龄增加所示。MRI可以获得可重复的总水和游离水的测量在体内的人类晶状体。观察到透镜稳态游离水梯度（但非总水梯度）随年龄的增长而消失，这一观察结果提出了蛋白质-水相互作用的改变是透镜光学性能和随年龄增长观察到的整体视力退化的潜在原因的可能性。
To use magnetic resonance imaging (MRI) to measure age-dependent changes in total and free water in human lenses in vivo. Sixty-four healthy adults aged 18 to 86 years were recruited, fitted with a 32-channel head receiver coil, and placed in a 3 Tesla clinical MR scanner. Scans of the crystalline lens were obtained using a volumetric interpolated breath-hold examination sequence with dual flip angles, which were corrected for field inhomogeneity post-acquisition using a B1-map obtained using a turbo-FLASH sequence. The spatial distribution and content of corrected total (ρlens) and free (T1) water along the lens optical axis were extracted using custom-written code. Lens total water distribution and content did not change with age (all P > 0.05). In contrast to total water, a gradient in free water content that was highest in the periphery relative to the center was present in lenses across all ages. However, this initially parabolic free water gradient gradually developed an enhanced central plateau, as indicated by increasing profile shape parameter values (anterior: 0.067/y, P = 0.004; posterior: 0.050/y, P = 0.020) and central free water content (1.932 ms/y, P = 0.022) with age. MRI can obtain repeatable total and free water measurements of in vivo human lenses. The observation that the lens steady-state free, but not total, water gradient is abolished with age raises the possibility that alterations in protein-water interactions are an underlying cause of the degradation in lens optics and overall vision observed with aging.
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影响因子：
3.4
作者：
Augusteyn, Robert C.
通讯作者：
Augusteyn, Robert C.
影响因子：
62.1
作者：
Bellissent-Funel MC;Hassanali A;Havenith M;Henchman R;Pohl P;Sterpone F;van der Spoel D;Xu Y;Garcia AE
通讯作者：
Garcia AE
影响因子：
1.8
作者：
Dubbelman, M;Van der Heijde, GL
通讯作者：
Van der Heijde, GL
影响因子：
3.3
作者：
Deoni, SCL;Rutt, BK;Peters, TM
通讯作者：
Peters, TM
影响因子：
3.3
作者：
Deoni, SCL;Peters, TM;Rutt, BK
通讯作者：
Rutt, BK