A Novel Suspended Hydrogel Membrane Platform for Cell Culture
A Novel Suspended Hydrogel Membrane Platform for Cell Culture
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用于细胞培养的新型悬浮水凝胶膜平台
DOI：
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
P. Soman
中科院分区：
文献类型：
--
作者：
Y. X. Chen;Shih;Jiahan Yan;M. Hsieh;Lingyan Weng;Jessica L. Ouderkirk;M. Krendel;P. Soman
目前的细胞培养主要在合成的二维 (2D) 培养皿或可渗透的支持物（如博伊登室）上进行，主要是因为它们易于使用且已建立协议。人们普遍认为，现代细胞生物学研究需要新的生理相关的三维（3D）细胞培养平台来模拟体内细胞反应。为此，我们报告了使用甲基丙烯酸明胶（GelMA）水凝胶设计和开发悬浮水凝胶膜（ShyM）平台。 ShyM 厚度 (0.25–1 mm) 和机械性能 (10–70 kPa) 可以通过分别控制支撑网格的尺寸和 GelMA 预聚物的浓度来改变。使用小鼠 10T1/2 细胞进行测试时发现，采用双介质暴露的 GelMA ShyM 与细胞接种和细胞封装方法兼容，并且与平坦的 GelMA 未悬浮对照相比，显示出更高的细胞扩散和增殖。 ShyM 的实用性还通过癌细胞侵袭的案例研究得到了证明。 ShyM 与 Boyden 室类似，与标准孔板设计兼容，并且可以使用常用的 3D 打印机进行打印。未来，ShyM 可以扩展到各种光敏水凝胶和细胞类型，开发新的体外测定法来研究复杂的细胞-细胞和细胞-细胞外基质 (ECM) 相互作用。
Current cell-culture is largely performed on synthetic two-dimensional (2D) petri dishes or permeable supports such as Boyden chambers, mostly because of their ease of use and established protocols. It is generally accepted that modern cell biology research requires new physiologically relevant three-dimensional (3D) cell culture platform to mimic in vivo cell responses. To that end, we report the design and development of a suspended hydrogel membrane (ShyM) platform using gelatin methacrylate (GelMA) hydrogel. ShyM thickness (0.25–1 mm) and mechanical properties (10–70 kPa) can be varied by controlling the size of the supporting grid and concentration of GelMA prepolymer, respectively. GelMA ShyMs, with dual media exposure, were found to be compatible with both the cell-seeding and the cell-encapsulation approach as tested using murine 10T1/2 cells and demonstrated higher cellular spreading and proliferation as compared to flat GelMA unsuspended control. The utility of ShyM was also demonstrated using a case-study of invasion of cancer cells. ShyMs, similar to Boyden chambers, are compatible with standard well-plates designs and can be printed using commonly available 3D printers. In the future, ShyM can be potentially extended to variety of photosensitive hydrogels and cell types, to develop new in vitro assays to investigate complex cell–cell and cell–extracellular matrix (ECM) interactions.
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