Inhibition of melanogenesis versus antioxidant properties of essential oil extracted from leaves of Vitex negundo Linn and chemical composition analysis by GC-MS.
Inhibition of melanogenesis versus antioxidant properties of essential oil extracted from leaves of Vitex negundo Linn and chemical composition analysis by GC-MS.
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从Vitex negundo linn叶片中提取的精油和GC-MS化学成分分析的精油的黑色素生成与抗氧化特性的抑制作用。
DOI：
10.3390/molecules17043902
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发表时间：
2012-03-30
期刊：
影响因子：
--
通讯作者：
Chang TM
中科院分区：
文献类型：
--
作者：
Huang HC;Chang TY;Chang LZ;Wang HF;Yih KH;Hsieh WY;Chang TM
本研究旨在研究从 V. negundo Linn 叶子中提取的精油的抗黑色素生成和抗氧化特性，并分析该精油的化学成分。通过分光光度法评估精油的功效，并通过气相色谱-质谱法（GC-MS）分析精油中的挥发性化合物。结果表明，精油能有效抑制小鼠 B16F10 酪氨酸酶活性，并以剂量依赖的方式减少黑色素的数量。此外，该精油可显着清除 2,2-二苯基-1-三硝基苯肼 (DPPH) 和 2,2'-连氮基-双(3-乙基苯并噻唑啉-6-磺酸) (ABTS) 自由基，并以剂量依赖性模式显示出与金属离子螯合特性相比的有效还原能力。精油的化学成分为倍半萜类(44.41%)、单萜类(19.25%)、酯类(14.77%)、醇类(8.53%)、芳香族化合物(5.90%)、酮类(4.96%)、醚类(0.4%)，合计占其化学成分的98.22%。据预测，精油中的芳香族化合物可能有助于其抗氧化活性。结果表明，从 V. negundo Linn 叶中提取的精油可减少 B16F10 黑色素瘤细胞中黑色素的产生，并显示出有效的抗氧化活性。因此，精油可以作为黑色素合成的抑制剂，也可以作为天然抗氧化剂。
This study was aimed at investigating the antimelanogenic and antioxidative properties of the essential oil extracted from leaves of V. negundo Linn and the analysis of the chemical composition of this essential oil. The efficacy of the essential oil was evaluated spectrophotometrically, whereas the volatile chemical compounds in the essential oil were analyzed by gas chromatography-mass spectrometry (GC-MS). The results revealed that the essential oil effectively suppresses murine B16F10 tyrosinase activity and decreases the amount of melanin in a dose-dependent manner. Additionally, the essential oil significantly scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals, and showed potent reducing power versus metal-ion chelating properties in a dose-dependent pattern. The chemical constituents in the essential oil are sesquiterpenes (44.41%), monoterpenes (19.25%), esters (14.77%), alcohols (8.53%), aromatic compound (5.90%), ketone (4.96%), ethers (0.4%) that together account for 98.22% of its chemical composition. It is predicted that the aromatic compound in the essential oil may contribute to its antioxidant activities. The results indicated that essential oil extracted from V. negundo Linn leaves decreased melanin production in B16F10 melanoma cells and showed potent antioxidant activities. The essential oil can thereby serve as an inhibitor of melanin synthesis and could also act as a natural antioxidant.
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影响因子：
9.3
作者：
Büyükokuroglu, ME;Gülçin, I;Küfrevioglu, Ö
通讯作者：
Küfrevioglu, Ö
影响因子：
5.4
作者：
Dharmasiri, MG;Jayakody, JRAC;Ratnasooriya, WD
通讯作者：
Ratnasooriya, WD
影响因子：
8.2
作者：
Yang, Jeong-yeh;Koo, Jeung-hyun;Park, Jin-woo
通讯作者：
Park, Jin-woo
DOI：
10.1016/s0021-9673(01)80947-x
发表时间：
1963-01-01
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
VANDENDOOL, H;KRATZ, PD
通讯作者：
KRATZ, PD
影响因子：
3.9
作者：
DINIS, TCP;MADEIRA, VMC;ALMEIDA, LM
通讯作者：
ALMEIDA, LM