Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.).
Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.).
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揭示萝卜（Raphanus sativus L.）根部蛋白质组变化及其与盐胁迫反应分子机制的关系
DOI：
10.3389/fpls.2017.01192
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发表时间：
2017
影响因子：
5.6
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Sun X;Wang Y;Xu L;Li C;Zhang W;Luo X;Jiang H;Liu L
为了解萝卜盐胁迫响应的分子机制，采用itraq蛋白组学分析方法研究了不同盐处理下萝卜蛋白质种类丰度的差异。CK与Na100、CK与Na200、Na100与Na200之间分别鉴定出851、706和685种差异丰度蛋白（DAPS）。功能注释分析表明，盐胁迫引起萝卜根系碳水化合物和能量代谢、蛋白质代谢、信号转导、转录调控、胁迫和防御运输等复杂的蛋白质组学改变。此外，利用RT-qPCR进一步验证了9个编码DAPS基因的表达水平。转录组学和蛋白质组学数据结合mirna进行了进一步的综合分析，以加强对萝卜对盐度反应的理解。负责信号转导、ROS清除和转运活性的基因以及miR171、miR395和miR398等几个关键mirna在萝卜盐胁迫响应中起着至关重要的作用。在此基础上，提出了盐胁迫响应的遗传调控网络示意图。本研究为了解萝卜根系盐胁迫反应的分子机制提供了有价值的见解，并将有助于开发有效的耐盐萝卜和其他根茎类蔬菜作物的基因工程策略。
To understand the molecular mechanism underlying salt stress response in radish, iTRAQ-based proteomic analysis was conducted to investigate the differences in protein species abundance under different salt treatments. In total, 851, 706, and 685 differential abundance protein species (DAPS) were identified between CK vs. Na100, CK vs. Na200, and Na100 vs. Na200, respectively. Functional annotation analysis revealed that salt stress elicited complex proteomic alterations in radish roots involved in carbohydrate and energy metabolism, protein metabolism, signal transduction, transcription regulation, stress and defense and transport. Additionally, the expression levels of nine genes encoding DAPS were further verified using RT-qPCR. The integrative analysis of transcriptomic and proteomic data in conjunction with miRNAs was further performed to strengthen the understanding of radish response to salinity. The genes responsible for signal transduction, ROS scavenging and transport activities as well as several key miRNAs including miR171, miR395, and miR398 played crucial roles in salt stress response in radish. Based on these findings, a schematic genetic regulatory network of salt stress response was proposed. This study provided valuable insights into the molecular mechanism underlying salt stress response in radish roots and would facilitate developing effective strategies toward genetically engineered salt-tolerant radish and other root vegetable crops.
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影响因子：
5.2
作者：
Kubala, Szymon;Garnczarska, Malgorzata;Quinet, Muriel
通讯作者：
Quinet, Muriel
DOI：
10.1093/dnares/dsu014
发表时间：
2014-10
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Kitashiba H;Li F;Hirakawa H;Kawanabe T;Zou Z;Hasegawa Y;Tonosaki K;Shirasawa S;Fukushima A;Yokoi S;Takahata Y;Kakizaki T;Ishida M;Okamoto S;Sakamoto K;Shirasawa K;Tabata S;Nishio T
通讯作者：
Nishio T
影响因子：
5.1
作者：
Konishi, H;Yamane, H;Komatsu, S
通讯作者：
Komatsu, S
影响因子：
4.9
作者：
Katsuhara, M;Koshio, K;Kasamo, K
通讯作者：
Kasamo, K
影响因子：
4.3
作者：
Jagadeeswaran, Guru;Saini, Ajay;Sunkar, Ramanjulu
通讯作者：
Sunkar, Ramanjulu