Neuropeptide Y stimulates food intake and regulates metabolism in grass carp, Ctenopharyngodon idellus
Neuropeptide Y stimulates food intake and regulates metabolism in grass carp, Ctenopharyngodon idellus
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神经肽 Y 刺激草鱼摄食并调节新陈代谢
DOI：
10.1016/j.aquaculture.2012.11.033
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发表时间：
2013-03
期刊：
影响因子：
4.5
通讯作者：
梁旭方
中科院分区：
文献类型：
--
作者：
梁旭方
神经肽Y(NPY)是哺乳动物摄食的重要调节因子。然而，NPY在鱼类摄食行为中的作用尚未完全清楚。本研究探讨了NPY在草鱼摄食量中的作用。GC-NPY基因全长797个碱基，由96个氨基酸组成的开放阅读框架，包括28个残基和36个残基的成熟肽，与鲤科鱼类的NPY有很高的同源性。在哺乳动物中介导NPY刺激食欲的两个受体Y1和Y5在一些Eutelost基因组中丢失。然而，在我们之前的研究中，其他NPY受体Y8a和Y8b的序列都是从草鱼的EST文库中获得的。NPY和两种Y8受体主要在脑内表达。在72小时的观察期内，脑室内注射NPY显著增加了动物的累积摄食量。为了更好地了解NPY在草鱼中的作用，我们对草鱼食欲调节基因、能量代谢基因和关键消化酶的mRNA水平进行了研究。结果表明，注射NPY后，Y8b基因的表达水平显著升高，而胆囊收缩素(CCK)、可卡因和苯丙胺调节转录物(CART)、垂体腺苷环化酶激活肽(PACAP)、瘦素、瘦素受体(Leptin R)和Leptin受体重叠转录蛋白(LEPROT)的表达显著降低。NPY对肝脏胰岛素样生长因子1(IGF1)、胰岛素样生长因子1受体(IGF1R)、脂蛋白脂酶(LPL)、硬脂酰辅酶A脱饱和酶1(SCD1)、淀粉酶和胰蛋白酶的mRNA水平有明显的刺激作用，解偶联蛋白1(UCP 1)的mRNA水平有下降趋势，但差异不显著。这些结果表明，NPY在草鱼体内是一种促食因子，Y8b可能参与了草鱼的摄食调节。NPY不仅影响草鱼的摄食量，而且对能量代谢也有显著影响。此外，NPY似乎与其他食欲调节因子密切相互作用，目前的结果为这些复杂的相互作用提供了新的见解。对鱼类食欲调节的进一步了解最终有可能产生提高水产养殖饲料效率的方法。
Neuropeptide Y (NPY) has been established as an important regulator of food intake in mammals. However, the involvement of NPY in the feeding behavior of fish has not yet been fully understood. The present study investigated the role of NPY in food intake in grass carp. The full-length cDNA sequence of gc-NPY was 797bp, and an ORF of 96 amino acids, including the 28-residue signal peptide and 36-residue mature peptide, which had high identities to the NPY of cyprinid fishes. Two receptors Y1 and Y5 which mediate the appetite-stimulating effects of NPY in mammals lost from some of the euteleost genomes. However, sequences of other NPY receptors Y8a and Y8b were obtained from the ESTs library of grass carp in our previous studies. NPY and both Y8 receptors were predominantly expressed in the brain. Cumulative food intake was significantly increased by intracerebroventricular administration of NPY during a 72-h observation period. For better understanding of NPY's action in grass carp, the mRNA levels of appetite regulators, energy metabolism genes and key digestive enzymes were assessed. The results showed that the mRNA levels of Y8b were significantly increased, while the expressions of cholecystokinin (CCK), cocaine and amphetamine regulated transcript (CART), pituitary adenylate cyclase-activating peptide (PACAP), leptin, leptin receptor (leptinR) and leptin receptor overlapping transcript protein (LEPROT) were significantly decreased after NPY injection. In addition, the marked stimulation of insulin-like growth factor1 (IGF1), insulin-like growth factor 1 receptor (IGF1R), lipoprotein lipase (LPL), Stearoyl-CoA desaturase 1 (SCD1), amylase and trypsin mRNA levels in liver were caused by NPY administration, and the mRNA level of uncoupling protein 1(UCP 1) tended to decrease but not significantly. These results indicate that NPY acts as an orexigenic factor in the grass carp, and Y8b may involve in the feeding regulation. NPY not only affects food intake, but also has marked effects on energy metabolism in grass carp. Moreover, NPY seems to closely interact with other appetite regulators, and the present results provide novel insight into these complex interactions. Further understandings of appetite regulation in fish ultimately have the potential to yield methods for improving feed efficiency in aquaculture.
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影响因子：
--
作者：
BILLINGTON, CJ;BRIGGS, JE;LEVINE, AS
通讯作者：
LEVINE, AS
影响因子：
2.7
作者：
A. Chiba
通讯作者：
A. Chiba
影响因子：
3.4
作者：
Larsson TA;Olsson F;Sundstrom G;Lundin LG;Brenner S;Venkatesh B;Larhammar D
通讯作者：
Larhammar D
DOI：
10.1016/j.cbpb.2006.11.009
发表时间：
2007-03
期刊：
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
影响因子：
--
作者：
K. Murashita;H. Fukada;H. Hosokawa;T. Masumoto
通讯作者：
K. Murashita;H. Fukada;H. Hosokawa;T. Masumoto
影响因子：
3.5
作者：
A. Lindberg;G. Olivecrona
通讯作者：
A. Lindberg;G. Olivecrona