Comparing the bacterial diversity of acute and chronic dental root canal infections.
Comparing the bacterial diversity of acute and chronic dental root canal infections.
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DOI：
10.1371/journal.pone.0028088
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Tiedje JM
中科院分区：
文献类型：
--
作者：
Santos AL;Siqueira JF Jr;Rôças IN;Jesus EC;Rosado AS;Tiedje JM
这项研究使用454FLX测序仪进行条形码多重焦磷酸测序，以比较与急性(有症状)或慢性(无症状)根尖周炎相关的牙根管感染的微生物区系。从9个急性脓肿和8个慢性感染的标本中分析得到部分16S rRNA基因序列，将其分类为916个细菌种水平操作分类单位(OTU)，隶属于13个门67属。在急性感染中，细菌数量最多的是细菌(52%)、梭杆菌(17%)和类杆菌(13%)，而在慢性感染中，优势菌是细菌(59%)、类杆菌(14%)和放线杆菌(10%)。梭菌属在急性病例(89%)比慢性病例(50%)更常见。急性感染中最丰富/最流行的属是梭杆菌和细小单胞菌。仅在急性感染中检出20属，在慢性感染中检出18属。在3%差异的OTUS中，只有18%(n = 165)由急性和慢性感染所共有。多样性和丰富度估计值显示，急性感染明显比慢性感染更加多样化。虽然观察到细菌群落的个体间差异很大，但许多样本往往根据感染类型(急性或慢性)分组在一起。这项研究是对与急性和慢性牙根管感染相关的微生物区系进行的最全面和最深入的比较之一，并强调了不同的多菌群落在急性感染中作为致病单位的作用。焦磷酸测序技术揭示的牙髓感染的总体多样性远远高于先前报告的牙髓感染。
This study performed barcoded multiplex pyrosequencing with a 454 FLX instrument to compare the microbiota of dental root canal infections associated with acute (symptomatic) or chronic (asymptomatic) apical periodontitis. Analysis of samples from 9 acute abscesses and 8 chronic infections yielded partial 16S rRNA gene sequences that were taxonomically classified into 916 bacterial species-level operational taxonomic units (OTUs) (at 3% divergence) belonging to 67 genera and 13 phyla. The most abundant phyla in acute infections were Firmicutes (52%), Fusobacteria (17%) and Bacteroidetes (13%), while in chronic infections the dominant were Firmicutes (59%), Bacteroidetes (14%) and Actinobacteria (10%). Members of Fusobacteria were much more prevalent in acute (89%) than in chronic cases (50%). The most abundant/prevalent genera in acute infections were Fusobacterium and Parvimonas. Twenty genera were exclusively detected in acute infections and 18 in chronic infections. Only 18% (n = 165) of the OTUs at 3% divergence were shared by acute and chronic infections. Diversity and richness estimators revealed that acute infections were significantly more diverse than chronic infections. Although a high interindividual variation in bacterial communities was observed, many samples tended to group together according to the type of infection (acute or chronic). This study is one of the most comprehensive in-deep comparisons of the microbiota associated with acute and chronic dental root canal infections and highlights the role of diverse polymicrobial communities as the unit of pathogenicity in acute infections. The overall diversity of endodontic infections as revealed by the pyrosequencing technique was much higher than previously reported for endodontic infections.
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