High cell density and high-resolution 3D bioprinting for fabricating vascularized tissues.
High cell density and high-resolution 3D bioprinting for fabricating vascularized tissues.
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DOI：
10.1126/sciadv.ade7923
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发表时间：
2023-02-22
期刊：
影响因子：
13.6
通讯作者：
Chen, Shaochen
中科院分区：
文献类型：
--
作者：
You, Shangting;Xiang, Yi;Hwang, Henry H.;Berry, David B.;Kiratitanaporn, Wisarut;Guan, Jiaao;Yao, Emmie;Tang, Min;Zhong, Zheng;Ma, Xinyue;Wangpraseurt, Daniel;Sun, Yazhi;Lu, Ting-yu;Chen, Shaochen
三维（3D）生物打印技术已成为制造3D工程组织的最流行的方法特别的，基于数字光处理的生物打印症状的3D生物打印会因光散射而增加的生物焦细胞密度增加。散射诱导的生物打印分辨率恶化。每毫升细胞密度在灌注培养系统中可行，在培养14天后观察到内皮化和血管生成。
具有高细胞密度和高分辨率的活组织可以打印3D。
Three-dimensional (3D) bioprinting techniques have emerged as the most popular methods to fabricate 3D-engineered tissues; however, there are challenges in simultaneously satisfying the requirements of high cell density (HCD), high cell viability, and fine fabrication resolution. In particular, bioprinting resolution of digital light processing–based 3D bioprinting suffers with increasing bioink cell density due to light scattering. We developed a novel approach to mitigate this scattering-induced deterioration of bioprinting resolution. The inclusion of iodixanol in the bioink enables a 10-fold reduction in light scattering and a substantial improvement in fabrication resolution for bioinks with an HCD. Fifty-micrometer fabrication resolution was achieved for a bioink with 0.1 billion per milliliter cell density. To showcase the potential application in tissue/organ 3D bioprinting, HCD thick tissues with fine vascular networks were fabricated. The tissues were viable in a perfusion culture system, with endothelialization and angiogenesis observed after 14 days of culture. Living tissues with high cell density and high resolution can be 3D printed.
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DOI：
10.1016/j.mtbio.2021.100162
发表时间：
2021-09
期刊：
Materials today. Bio
影响因子：
--
作者：
Levato R;Lim KS;Li W;Asua AU;Peña LB;Wang M;Falandt M;Bernal PN;Gawlitta D;Zhang YS;Woodfield TBF;Malda J
通讯作者：
Malda J
影响因子：
7.7
作者：
Boothe, Tobias;Hilbert, Lennart;Rink, Jochen C.
通讯作者：
Rink, Jochen C.
DOI：
10.1126/science.aav9750
发表时间：
2019-05-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Grigoryan B;Paulsen SJ;Corbett DC;Sazer DW;Fortin CL;Zaita AJ;Greenfield PT;Calafat NJ;Gounley JP;Ta AH;Johansson F;Randles A;Rosenkrantz JE;Louis-Rosenberg JD;Galie PA;Stevens KR;Miller JS
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Miller JS
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影响因子：
5.6
作者：
Guo Y;Li W;Qian M;Jiang T;Guo P;Du Q;Lin N;Xie X;Wu Z;Lin D;Liu D
通讯作者：
Liu D
影响因子：
5.9
作者：
Huang NF;Serpooshan V;Morris VB;Sayed N;Pardon G;Abilez OJ;Nakayama KH;Pruitt BL;Wu SM;Yoon YS;Zhang J;Wu JC
通讯作者：
Wu JC