High-resolution lithographic biofabrication of hydrogels with complex microchannels from low-temperature-soluble gelatin bioresins.
High-resolution lithographic biofabrication of hydrogels with complex microchannels from low-temperature-soluble gelatin bioresins.
复制标题
用低温可溶性明胶生物树脂制备具有复杂微通道的水凝胶的高分辨率光刻生物制造。
DOI：
10.1016/j.mtbio.2021.100162
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Malda J
中科院分区：
文献类型：
--
作者：
Levato R;Lim KS;Li W;Asua AU;Peña LB;Wang M;Falandt M;Bernal PN;Gawlitta D;Zhang YS;Woodfield TBF;Malda J
与传统的挤出技术相比，通过基于光的 3D 打印进行生物制造可提供卓越的分辨率和生成自由形状结构的能力。尽管在新型水凝胶生物墨水设计方面的广泛努力导致了挤出方法的重大进步，但平版生物打印的可及性仍然受到细胞友好树脂选择有限的阻碍。在此，我们报告了一套源自鱼源明胶的新型光响应生物树脂的开发，旨在打印具有嵌入式血管模拟通道的卷积网络的高分辨率水凝胶结构。与哺乳动物明胶不同，这些材料在室温下表现出作为预水凝胶溶液的热稳定性，非常适合在任何易于使用的平版打印机上进行生物打印。明胶骨架的降冰片烯和甲基丙烯酰修饰，与基于钌的可见光光引发剂和作为细胞相容性光吸收剂的新型椰壳碱相结合，可以打印具有高形状保真度的单像素特征（〜50μm）的结构，即使使用低硬度凝胶，也是细胞封装（1-2kPa）的理想选择。此外，水性两相乳液生物树脂可以调节印刷水凝胶本体的渗透性。生物打印的间充质基质细胞经过一个月的培养显示出高功能性，并经历了多谱系分化，同时用组织特异性的新沉积的细胞外基质定植于生物树脂块中。 Importantly, printed hydrogels embedding complex channels with perfusable lumen (diameter <200 μm) were obtained, replicating anatomical 3D networks with out-of-plane branches (i.e. brain vessels) that cannot otherwise be reproduced by extrusion bioprinting.这种多功能生物树脂平台为广泛采用光刻生物制造以及生物打印复杂的通道负载结构开辟了新途径，并在再生医学和基于水凝胶的器官芯片设备中具有预期的应用。
Biofabrication via light-based 3D printing offers superior resolution and ability to generate free-form architectures, compared to conventional extrusion technologies. While extensive efforts in the design of new hydrogel bioinks lead to major advances in extrusion methods, the accessibility of lithographic bioprinting is still hampered by a limited choice of cell-friendly resins. Herein, we report the development of a novel set of photoresponsive bioresins derived from ichthyic-origin gelatin, designed to print high-resolution hydrogel constructs with embedded convoluted networks of vessel-mimetic channels. Unlike mammalian gelatins, these materials display thermal stability as pre-hydrogel solutions at room temperature, ideal for bioprinting on any easily-accessible lithographic printer. Norbornene- and methacryloyl-modification of the gelatin backbone, combined with a ruthenium-based visible light photoinitiator and new coccine as a cytocompatible photoabsorber, allowed to print structures resolving single-pixel features (∼50 μm) with high shape fidelity, even when using low stiffness gels, ideal for cell encapsulation (1–2 kPa). Moreover, aqueous two-phase emulsion bioresins allowed to modulate the permeability of the printed hydrogel bulk. Bioprinted mesenchymal stromal cells displayed high functionality over a month of culture, and underwent multi-lineage differentiation while colonizing the bioresin bulk with tissue-specific neo-deposited extracellular matrix. Importantly, printed hydrogels embedding complex channels with perfusable lumen (diameter <200 μm) were obtained, replicating anatomical 3D networks with out-of-plane branches (i.e. brain vessels) that cannot otherwise be reproduced by extrusion bioprinting. This versatile bioresin platform opens new avenues for the widespread adoption of lithographic biofabrication, and for bioprinting complex channel-laden constructs with envisioned applications in regenerative medicine and hydrogel-based organ-on-a-chip devices.
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影响因子：
16.6
作者：
Kim SH;Yeon YK;Lee JM;Chao JR;Lee YJ;Seo YB;Sultan MT;Lee OJ;Lee JS;Yoon SI;Hong IS;Khang G;Lee SJ;Yoo JJ;Park CH
通讯作者：
Park CH
影响因子：
6.8
作者：
Kuo, Alexandra P.;Bhattacharjee, Nirveek;Folch, Albert
通讯作者：
Folch, Albert
DOI：
10.1126/science.aav9750
发表时间：
2019-05-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Grigoryan B;Paulsen SJ;Corbett DC;Sazer DW;Fortin CL;Zaita AJ;Greenfield PT;Calafat NJ;Gounley JP;Ta AH;Johansson F;Randles A;Rosenkrantz JE;Louis-Rosenberg JD;Galie PA;Stevens KR;Miller JS
通讯作者：
Miller JS
生物学家的生物印刷。
影响因子：
64.5
作者：
Daly, Andrew C.;Prendergast, Margaret E.;Hughes, Alex J.;Burdick, Jason A.
通讯作者：
Burdick, Jason A.
影响因子：
3.8
作者：
Chimene, David;Lennox, Kimberly K.;Gaharwar, Akhilesh K.
通讯作者：
Gaharwar, Akhilesh K.