Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy.
Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy.
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通过相关的体内生理学、同步加速器显微断层扫描和体积电子显微镜对脑组织进行功能和多尺度3D结构研究。
DOI：
10.1038/s41467-022-30199-6
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发表时间：
2022-05-25
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
了解生物组织的功能需要对生理学和结构进行协调的研究，探索包含完整功能单元的体积，并详细解析相关特征。在这里，我们介绍了一种方法来解决这一挑战：小鼠脑组织切片包含一个区域，其中使用在体内双光子钙成像记录功能进行染色，脱水，树脂包埋和同步辐射X射线计算机断层扫描与传播相衬（SXRT）成像。SXRT提供了亚细胞细节的背景，并且可以使用连续块面电子显微镜（SBEM）进行多体积的靶向采集。在嗅球中，结合SXRT和SBEM能够消除体内指定的感兴趣区域的歧义。海马CA1a区浅层锥体神经元棘器密度较深层大。总之，这种方法可以使亚细胞功能和结构的调查中的细胞和组织的背景下。生物组织的功能在其生理和结构中被编码。在这里，Bosch等人通过结合体内光、同步加速器X射线和体积电子显微镜来整合这两种见解来研究特定的神经元回路。
Understanding the function of biological tissues requires a coordinated study of physiology and structure, exploring volumes that contain complete functional units at a detail that resolves the relevant features. Here, we introduce an approach to address this challenge: Mouse brain tissue sections containing a region where function was recorded using in vivo 2-photon calcium imaging were stained, dehydrated, resin-embedded and imaged with synchrotron X-ray computed tomography with propagation-based phase contrast (SXRT). SXRT provided context at subcellular detail, and could be followed by targeted acquisition of multiple volumes using serial block-face electron microscopy (SBEM). In the olfactory bulb, combining SXRT and SBEM enabled disambiguation of in vivo-assigned regions of interest. In the hippocampus, we found that superficial pyramidal neurons in CA1a displayed a larger density of spine apparati than deeper ones. Altogether, this approach can enable a functional and structural investigation of subcellular features in the context of cells and tissues. The function of biological tissues is encoded in their physiology and structure. Here, Bosch et al. have integrated both insights to study specific neuronal circuits by combining in vivo light, synchrotron X-ray and volume electron microscopy.
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影响因子：
16.6
作者：
Arneodo EM;Penikis KB;Rabinowitz N;Licata A;Cichy A;Zhang J;Bozza T;Rinberg D
通讯作者：
Rinberg D
DOI：
10.1073/pnas.2012533117
发表时间：
2020-12-29
影响因子：
11.1
作者：
Andersson M;Kjer HM;Rafael-Patino J;Pacureanu A;Pakkenberg B;Thiran JP;Ptito M;Bech M;Bjorholm Dahl A;Andersen Dahl V;Dyrby TB
通讯作者：
Dyrby TB
影响因子：
64.8
作者：
Ghosh, Sulagna;Larson, Stephen D.;Baldwin, Kristin K.
通讯作者：
Baldwin, Kristin K.
影响因子：
64.8
作者：
Ding H;Smith RG;Poleg-Polsky A;Diamond JS;Briggman KL
通讯作者：
Briggman KL
影响因子：
48
作者：
Boergens, Kevin M.;Berning, Manuel;Helmstaedter, Moritz
通讯作者：
Helmstaedter, Moritz