Rabies virus glycoprotein as a carrier for anthrax protective antigen.
Rabies virus glycoprotein as a carrier for anthrax protective antigen.
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狂犬病病毒糖蛋白作为炭疽抗原的载体。
DOI：
10.1016/j.virol.2006.05.010
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发表时间：
2006-09-30
期刊：
影响因子：
3.7
通讯作者：
Schnell, Matthias J.
中科院分区：
文献类型：
--
作者：
Smith, Mary Ellen;Koser, Martin;Xiao, Sa;Siler, Catherine;McGettigan, James P.;Calkins, Catherine;Pomerantz, Roger J.;Dietzschold, Bernhard;Schnell, Matthias J.
Live viral vectors expressing foreign antigens have shown great promise as vaccines against viral diseases. However, safety concerns remain a major problem regarding the use of even highly attenuated viral vectors. Using the rabies virus (RV) envelope protein as a carrier molecule, we show here that inactivated RV particles can be utilized to present Bacillus anthracis protective antigen (PA) domain-4 in the viral membrane. In addition to the RV glycoprotein (G) transmembrane and cytoplasmic domains, a portion of the RV G ectodomain was required to express the chimeric RV G anthrax PA on the cell surface. The novel antigen was also efficiently incorporated into RV virions. Mice immunized with the inactivated recombinant RV virions exhibited seroconversion against both RV G and anthrax PA, and a second inoculation greatly increased these responses. These data demonstrate that a viral envelope protein can carry a bacterial protein and that a viral carrier can display whole polypeptides compared to the limited epitope presentation of previous viral systems.
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影响因子：
3.7
作者：
Gaudin Y;Ruigrok RW;Tuffereau C;Knossow M;Flamand A
通讯作者：
Flamand A
影响因子：
5.5
作者：
Beedham, RJ;Turnbull, PCB;Williamson, ED
通讯作者：
Williamson, ED
影响因子：
82.9
作者：
Fehr, T;Naim, HY;Zinkernagel, RM
通讯作者：
Zinkernagel, RM
影响因子：
3.1
作者：
Flick-Smith, HC;Walker, NJ;Williamson, ED
通讯作者：
Williamson, ED
影响因子：
5.5
作者：
Haupt, W
通讯作者：
Haupt, W