Arousal state transitions occlude sensory-evoked neurovascular coupling in neonatal mice.
Arousal state transitions occlude sensory-evoked neurovascular coupling in neonatal mice.
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DOI：
10.1038/s42003-023-05121-5
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发表时间：
2023-07-17
影响因子：
5.9
通讯作者：
Drew, Patrick J. J.
中科院分区：
文献类型：
--
作者：
Gheres, Kyle W. W.;Unsal, Hayreddin S. S.;Han, Xu;Zhang, Qingguang;Turner, Kevin L. L.;Zhang, Nanyin;Drew, Patrick J. J.
在成人感觉皮层，感觉刺激引起的神经活动增加通常由神经血管偶联介导的血管扩张。然而，新生动物的神经血管偶联是否与成年动物相同是有争议的，因为已经观察到了规范和倒置反应。我们利用光学成像、电生理学和BOLD功能磁共振成像技术研究了未麻醉新生小鼠神经血管偶联的本质。我们发现，在新生(出生后第15天，第15天)小鼠中，感觉刺激会导致血容量的小幅增加/BOLD信号，随后往往是血容量的大幅减少。对小鼠唤醒状态的检查显示，新生小鼠大部分时间都处于睡眠状态，而这种刺激会导致动物醒来。由于快速眼动和非快速眼动睡眠期间的皮质血容量比清醒状态高得多，觉醒会阻断任何感觉诱发的神经血管偶联。当新生小鼠在清醒期间受到刺激时，它们表现出相对正常(但放缓)的神经血管耦合，表明通常观察到的收缩是由于唤醒状态的变化。这些结果表明，与睡眠相关的血管变化主导着任何感觉诱发的变化，血流动力学措施需要在觉醒状态变化的背景下考虑。在未麻醉的新生小鼠中，结合光学成像、电生理学和BOLD功能磁共振成像显示，与睡眠相关的血管变化主导于感觉诱发的变化。
In the adult sensory cortex, increases in neural activity elicited by sensory stimulation usually drive vasodilation mediated by neurovascular coupling. However, whether neurovascular coupling is the same in neonatal animals as adults is controversial, as both canonical and inverted responses have been observed. We investigated the nature of neurovascular coupling in unanesthetized neonatal mice using optical imaging, electrophysiology, and BOLD fMRI. We find in neonatal (postnatal day 15, P15) mice, sensory stimulation induces a small increase in blood volume/BOLD signal, often followed by a large decrease in blood volume. An examination of arousal state of the mice revealed that neonatal mice were asleep a substantial fraction of the time, and that stimulation caused the animal to awaken. As cortical blood volume is much higher during REM and NREM sleep than the awake state, awakening occludes any sensory-evoked neurovascular coupling. When neonatal mice are stimulated during an awake period, they showed relatively normal (but slowed) neurovascular coupling, showing that that the typically observed constriction is due to arousal state changes. These result show that sleep-related vascular changes dominate over any sensory-evoked changes, and hemodynamic measures need to be considered in the context of arousal state changes. A combination of optical imaging, electrophysiology, and BOLD fMRI in unanesthetized neonatal mice reveals that sleep-related vascular changes dominate over sensory-evoked changes.
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DOI：
10.1177/1073858418805427
发表时间：
2019-08
期刊：
The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry
影响因子：
--
作者：
Drew PJ;Winder AT;Zhang Q
通讯作者：
Zhang Q
晶须在C57BL/6小鼠的感觉运动发展中的作用。
影响因子：
2.7
作者：
Arakawa, Hiroyuki;Erzurumlu, Reha S.
通讯作者：
Erzurumlu, Reha S.
影响因子：
4.6
作者：
Adams MD;Winder AT;Blinder P;Drew PJ
通讯作者：
Drew PJ
影响因子：
48
作者：
Drew, Patrick J.;Shih, Andy Y.;Driscoll, Jonathan D.;Knutsen, Per Magne;Blinder, Pablo;Davalos, Dimitrios;Akassoglou, Katerina;Tsai, Philbert S.;Kleinfeld, David
通讯作者：
Kleinfeld, David
影响因子：
6.3
作者：
Bekar, Lane K.;Wei, Helen S.;Nedergaard, Maiken
通讯作者：
Nedergaard, Maiken