Chronic ethanol consumption profoundly alters regional brain ceramide and sphingomyelin content in rodents.
Chronic ethanol consumption profoundly alters regional brain ceramide and sphingomyelin content in rodents.
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DOI：
10.1021/cn500174c
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发表时间：
2015-02-18
影响因子：
5
通讯作者：
Woods, Amina S.
中科院分区：
文献类型：
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作者：
Roux, Aurelie;Muller, Ludovic;Jackson, Shelley N.;Baldwin, Katherine;Womack, Virginia;Pagiazitis, John G.;O'Rourke, Joseph R.;Thanos, Panayotis K.;Balaban, Carey;Schultz, J. Albert;Volkow, Nora D.;Woods, Amina S.
关键词：
神经酰胺（CER）参与酒精诱导的神经炎症。在小鼠模型的慢性酒精暴露，16 CER和18鞘磷脂（SM）的浓度，从全脑脂质提取物使用电喷雾质谱法进行了测量。在酒精暴露的成年人中，所有18种CER浓度均显著升高（范围：25-607%）;在青少年中，6种CER浓度降低（范围：-9至37%）。与此相反，只有三个SM在成人和一个增加显着增加在少年。接下来，通过注入银纳米颗粒基质，然后进行聚焦激光解吸，利用基质注入激光解吸/电离质谱成像（MILDI-MSI）从冠状切片获得50 μm空间分辨率的区域识别。在全脑提取物中定量的大多数CER和SM在MILDI图像中被检测到。从三个大脑水平的冠状切片显示CER-SM离子强度的定性区域变化，作为组和脑区域的函数，在皮层，纹状体，缰核，海马。某些白色物质CER-SM对的高度相关变化发生在所有组的区域，包括成年小鼠的海马和外侧（但不是内侧）小脑皮质。我们的数据提供了第一个微尺度MS证据区域脂质强度变化引起的酒精。
Ceramides (CER) are involved in alcohol-induced neuroinflammation. In a mouse model of chronic alcohol exposure, 16 CER and 18 sphingomyelin (SM) concentrations from whole brain lipid extracts were measured using electrospray mass spectrometry. All 18 CER concentrations in alcohol exposed adults increased significantly (range: 25–607%); in juveniles, 6 CER decreased (range: −9 to −37%). In contrast, only three SM decreased in adult and one increased significantly in juvenile. Next, regional identification at 50 μm spatial resolution from coronal sections was obtained with matrix implanted laser desorption/ionization mass spectrometry imaging (MILDI-MSI) by implanting silver nanoparticulate matrices followed by focused laser desorption. Most of the CER and SM quantified in whole brain extracts were detected in MILDI images. Coronal sections from three brain levels show qualitative regional changes in CER-SM ion intensities, as a function of group and brain region, in cortex, striatum, accumbens, habenula, and hippocampus. Highly correlated changes in certain white matter CER-SM pairs occur in regions across all groups, including the hippocampus and the lateral (but not medial) cerebellar cortex of adult mice. Our data provide the first microscale MS evidence of regional lipid intensity variations induced by alcohol.
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影响因子：
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作者：
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通讯作者：
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