A large, switchable optical clearing skull window for cerebrovascular imaging.
A large, switchable optical clearing skull window for cerebrovascular imaging.
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作者：
Zhang C;Feng W;Zhao Y;Yu T;Li P;Xu T;Luo Q;Zhu D
原理：活体光学成像是研究脑血管结构和功能的重要方法。然而，其成像对比度和深度受到混浊颅骨的限制。组织光学透明具有解决这一问题的巨大潜力。我们的目标是开发一个透明的颅骨窗，而不进行开颅手术，用于评估脑血管结构和功能。研究方法：将颅骨光学透明剂局部应用于小鼠颅骨，在15 min内创建透明窗口。然后研究颅骨窗口的透明功效、可重复性和安全性。结果：光学透明颅骨窗成像增强了活体图像的对比度和深度。颅骨窗可用于2-8月龄小鼠，并可从局部扩大到双半球。此外，可以重复建立窗口，而不会诱导可观察到的炎症和代谢毒性。结论：我们成功地开发了一种易于操作、大、可切换且安全的光学透明颅骨窗。结合各种光学成像技术，可以通过这种光学透明颅骨窗观察脑血管的结构和功能。因此，它有可能用于基础研究的皮质血管的病理生理过程。
Rationale: Intravital optical imaging is a significant method for investigating cerebrovascular structure and function. However, its imaging contrast and depth are limited by the turbid skull. Tissue optical clearing has a great potential for solving this problem. Our goal was to develop a transparent skull window, without performing a craniotomy, for use in assessing cerebrovascular structure and function. Methods: Skull optical clearing agents were topically applied to the skulls of mice to create a transparent window within 15 min. The clearing efficacy, repeatability, and safety of the skull window were then investigated. Results: Imaging through the optical clearing skull window enhanced both the contrast and the depth of intravital imaging. The skull window could be used on 2-8-month-old mice and could be expanded from regional to bi-hemispheric. In addition, the window could be repeatedly established without inducing observable inflammation and metabolic toxicity. Conclusion: We successfully developed an easy-to-handle, large, switchable, and safe optical clearing skull window. Combined with various optical imaging techniques, cerebrovascular structure and function can be observed through this optical clearing skull window. Thus, it has the potential for use in basic research on the physiopathologic processes of cortical vessels.
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影响因子：
14.8
作者：
Holtmaat, Anthony;Bonhoeffer, Tobias;Chow, David K.;Chuckowree, Jyoti;De Paola, Vincenzo;Hofer, Sonja B.;Huebener, Mark;Keck, Tara;Knott, Graham;Lee, Wei-Chung A.;Mostany, Ricardo;Mrsic-Flogel, Tom D.;Nedivi, Elly;Portera-Cailliau, Carlos;Svoboda, Karel;Trachtenberg, Joshua T.;Wilbrecht, Linda
通讯作者：
Wilbrecht, Linda
影响因子：
16.2
作者：
Makino H;Ren C;Liu H;Kim AN;Kondapaneni N;Liu X;Kuzum D;Komiyama T
通讯作者：
Komiyama T
影响因子：
12.4
作者：
Huang Y;Lui H;Zhao J;Wu Z;Zeng H
通讯作者：
Zeng H
影响因子：
48
作者：
Drew, Patrick J.;Shih, Andy Y.;Driscoll, Jonathan D.;Knutsen, Per Magne;Blinder, Pablo;Davalos, Dimitrios;Akassoglou, Katerina;Tsai, Philbert S.;Kleinfeld, David
通讯作者：
Kleinfeld, David
影响因子：
56.9
作者：
Nimmerjahn, A;Kirchhoff, F;Helmchen, F
通讯作者：
Helmchen, F