Involvement of non-structural proteins (NS) in influenza A infection and viral tropism.
Involvement of non-structural proteins (NS) in influenza A infection and viral tropism.
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DOI：
10.1016/j.bbrc.2012.10.006
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发表时间：
2012-11-09
影响因子：
3.1
通讯作者：
Pang, Shen
中科院分区：
文献类型：
--
作者：
Yan, Xinmin;Wang, Qiuwei;Zhang, Zhiqing;Xie, Yiming;Zhang, Hong;Razi, Miriam;Hu, Shen;Zhang, Lijun;Cheng, Jinluo;Pang, Shen
甲型流感的血凝素(HA)已被报道为病毒感染的关键蛋白。因此，病毒包膜中该蛋白的密度和动态模式将影响病毒感染靶细胞。我们使用慢病毒系统来研究甲型H1N1流感病毒感染。在这里，我们证明了流感非结构蛋白(NS)显著促进病毒感染。用另一株H1N1亚株China246的NS基因片段替换A/WSN/1933 H1N1基因组中的NS基因片段，发现病毒的感染嗜性发生了显著变化。重组H1N1病毒与其亲本病毒A/WSN/1933相比，对人上皮细胞株HOT具有几乎相同的感染性，但在感染Madin-Darby犬肾(MDCK)细胞系时，其感染性仅为其亲本病毒的1/100。这些结果表明，NS不仅对人类流感病毒的传染性很重要，而且可能在病毒趋向性方面发挥关键作用，使病毒发生变异并传播到其他物种。
Hemagglutinin (HA) of influenza A has been reported as the key protein in viral infection. Therefore, the density and the dynamic pattern of this protein in viral envelope will affect the virus to infect target cells. We used a lentiviral system to study the influenza A H1N1 viral infection. Herein we demonstrate that the influenza non-structural proteins (NS) significantly promote viral infection. By substituting NS gene segment from an H1N1 genome set of A/WSN/1933 with the NS segment isolated from another H1N1 substrain genome set, China246, we found that viral infection tropism was significantly altered. The reassortant H1N1 shows almost identical infectivity compared with its parental virus, A/WSN/1933, for the human epithelial cell line HOT, but shows only 1/100 infectivity of its parental virus when infecting the Madin-Darby canine kidney (MDCK) cell line. These results suggest that not only is NS important in the infectivity of human influenza virus, but that it may play a critical role in viral tropism, allowing the virus to mutate and spread to other species.
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DOI：
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