Fluorescent indicators for imaging membrane potential of organelles.

Fluorescent indicators for imaging membrane potential of organelles.
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DOI:
10.1016/j.cbpa.2022.102203
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发表时间:
2022-12
影响因子:
7.8
通讯作者:
--
中科院分区:
生物学2区
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--
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Plasma membrane potential is a key driver of the physiology of excitable cells like neurons and cardiomyocytes. Voltage-sensitive fluorescent indicators offer a powerful complement to traditional electrode-based approaches to measuring and monitoring membrane potential. Intracellular organelles can also generate membrane potential, yet the electrode- and fluorescent indicator-based approaches used for plasma membrane potential imaging are difficult to implement on intact organelles in their native environment. Here, we survey recent advances in developing and deploying voltage-sensitive fluorescent indicators to interrogate organelle membrane potential in intact cells.
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