Lipidomics of oxidized polyunsaturated fatty acids.
Lipidomics of oxidized polyunsaturated fatty acids.
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DOI：
10.1016/j.freeradbiomed.2012.08.565
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发表时间：
2013-06
影响因子：
7.4
通讯作者：
Nicolaou, Anna
中科院分区：
文献类型：
--
作者：
Massey, Karen A.;Nicolaou, Anna
关键词：
脂质介体是由多不饱和脂肪酸通过酶和自由基介导氧化反应产生的。当通过环氧合酶、脂氧合酶和细胞色素P450单加氧酶进行氧化时，多不饱和脂肪酸可以产生一系列代谢物，包括二十烷类化合物、二十二烷类化合物和十八烷类化合物。这些有效的生物活性脂质参与了许多生化和信号通路，其中炎症尤为重要。此外，由于它们是由不止一种途径和底物产生的，并且存在于各种生物环境中，它们的分析并不总是可以用传统的分析方法进行。液相色谱结合电喷雾质谱仪为生物活性脂类的分析提供了一种通用和灵敏的方法，允许对同一样品中存在的多种物种进行特定和准确的定量。在这里，我们解释了介体脂质组学的这种方法的原理，并提出了分析多不饱和脂肪酸的酶产生的氧化代谢物的详细方案，这些代谢物可以被量身定做来回答生物学问题或促进营养和药物干预的评估。►多不饱和脂肪酸氧化为二十烷类、二十二烷类和十八烷类化合物。►我们用液相色谱-串联质谱法进行了脂类的同步分析。在各种生物基质中均可检测到低皮克/毫升的►多种脂类介体。►介体脂质组学与生物学、营养学和药理学研究相关。
Lipid mediators are produced from the oxidation of polyunsaturated fatty acids through enzymatic and free radical-mediated reactions. When subject to oxygenation via cyclooxygenases, lipoxygenases, and cytochrome P450 monooxygenases, polyunsaturated fatty acids give rise to an array of metabolites including eicosanoids, docosanoids, and octadecanoids. These potent bioactive lipids are involved in many biochemical and signaling pathways, with inflammation being of particular importance. Moreover, because they are produced by more than one pathway and substrate, and are present in a variety of biological milieus, their analysis is not always possible with conventional assays. Liquid chromatography coupled to electrospray mass spectrometry offers a versatile and sensitive approach for the analysis of bioactive lipids, allowing specific and accurate quantitation of multiple species present in the same sample. Here we explain the principles of this approach to mediator lipidomics and present detailed protocols for the assay of enzymatically produced oxygenated metabolites of polyunsaturated fatty acids that can be tailored to answer biological questions or facilitate assessment of nutritional and pharmacological interventions. ► Polyunsaturated fatty acids oxygenate to eicosanoids, docosanoids, and octadecanoids. ► We performed simultaneous analysis of lipids by liquid chromatography–tandem mass spectrometry. ► Multiple lipid mediators were detected at low picograms per milliliter and in various biological matrices. ► Mediator lipidomics are relevant to biological, nutritional, and pharmacological studies.
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影响因子：
13.6
作者：
Dobrian, Anca D.;Lieb, David C.;Cole, Banumathi K.;Taylor-Fishwick, David A.;Chakrabarti, Swarup K.;Nadler, Jerry L.
通讯作者：
Nadler, Jerry L.
影响因子：
7.1
作者：
Calder, Philip C.
通讯作者：
Calder, Philip C.
DOI：
10.1016/j.prostaglandins.2010.12.005
发表时间：
2011-04-01
影响因子：
2.9
作者：
Hossain, Mohammad Salim;Chowdhury, Abu Asad;Yokota, Kazushige
通讯作者：
Yokota, Kazushige
DOI：
10.1016/j.plefa.2009.11.002
发表时间：
2010-01-01
影响因子：
3
作者：
Durn, J. H.;Marshall, K. M.;Nicolaou, A.
通讯作者：
Nicolaou, A.
影响因子：
2
作者：
Bastani, Nasser E.;Gundersen, Thomas E.;Blomhoff, Rune
通讯作者：
Blomhoff, Rune