Modular poly(ethylene glycol) scaffolds provide the ability to decouple the effects of stiffness and protein concentration on PC12 cells.
Modular poly(ethylene glycol) scaffolds provide the ability to decouple the effects of stiffness and protein concentration on PC12 cells.
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DOI：
10.1016/j.actbio.2011.06.054
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发表时间：
2011-11
影响因子：
9.7
通讯作者：
Willits, Rebecca Kuntz
中科院分区：
文献类型：
--
作者：
Scott, Rebecca A.;Elbert, Donald L.;Willits, Rebecca Kuntz
本研究致力于开发一种由聚乙二醇微凝胶和I型胶原组装而成的模块化聚乙二醇支架，以提供一个在三维支架内分离化学和机械线索的环境。我们首先对微凝胶的制备过程进行了表征，检测了聚合物颗粒的大小、多分散性、溶胀率、网目大小和储存模数。得到的微凝胶具有低的多分散性，pDI值为1.08，直径约为1.6μm。根据溶胀比计算，微凝胶的网目尺寸为47.53o。然后将EDC/NHS活化的微凝胶与聚乙二醇4-芳胺和0、1、10或100μg/mL胶原压实，形成模块化水凝胶(ModuGel)。模型凝胶的硬度(G*)不会因添加胶原蛋白而显著改变，从而允许改变化学环境，而不依赖于支架的机械性能。随着胶原浓度的增加，模型凝胶中PC12细胞的聚集性增加，模型凝胶中的细胞存活率比块状聚乙二醇水凝胶中的细胞活力得到改善。总体而言，这些结果表明，进一步探索由微凝胶形成的模块化支架可以更好地理解化学和机械性能与细胞行为之间的关系。
This research focused on developing a modular poly(ethylene glycol) (PEG) scaffold, assembled from PEG microgels and collagen I, to provide an environment to decouple the chemical and mechanical cues within a three dimensional scaffold. We first characterized the microgel fabrication process, examining the size, polydispersity, swelling ratio, mesh size, and storage modulus of the polymer particles. The resulting microgels had a low polydispersity, PDI=1.08, and a diameter of ~1.6 μm. The mesh size of the microgels, calculated from the swelling ratio, was 47.53 Å. Modular hydrogels (modugels) were then formed by compacting EDC/NHS activated microgels with PEG-4arm-amine and 0, 1, 10, or 100 μg/mL collagen. Stiffness (G*) of the modugels was not significantly altered with the addition of collagen, allowing for modification of the chemical environment independent from the mechanical properties of the scaffold. PC12 cell aggregation increased in modugels as collagen concentrations increased and cell viability in modugels was improved over bulk PEG hydrogels. Overall, these results indicate that further exploration of modular scaffolds formed from microgels could allow for a better understanding of the relationship between the chemical and mechanical properties and cellular behavior.
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