Interspecific hybridization yields strategy for South Pacific filariasis vector elimination.
Interspecific hybridization yields strategy for South Pacific filariasis vector elimination.
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DOI：
10.1371/journal.pntd.0000129
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发表时间：
2008-01-16
影响因子：
3.8
通讯作者：
Dobson SL
中科院分区：
文献类型：
--
作者：
Brelsfoard CL;Séchan Y;Dobson SL
淋巴丝虫病（LF）是南太平洋地区残疾的主要原因，其中170万人口中有96%以上处于LF感染的风险中。作为当前全球运动的一部分，大规模药物管理（MDA）有效地降低了淋巴丝虫病的患病率，但蚊媒生物学可能使MDA策略复杂化。在某些地区，有证据表明，仅通过MDA无法实现消除LF的目标。专性媒介蚊子为打破LF传播周期提供了额外的目标，但现有的方法对于控制南太平洋大部分地区的主要媒介波利尼西亚伊蚊是无效的。在这里，我们证明了种间杂交和渐渗的结果，在一个A。波利尼西亚伊蚊菌株（“CP”菌株），其被来自河伊蚊的内共生沃尔巴克氏体细菌稳定感染。CP菌株与自然感染的蚊子双向不相容，导致雌性不育。实验室测定表明，CP雄性具有同等的竞争力，当CP雄性引入野生型A时，导致种群消除。波利尼西亚人研究结果表明，战略的可行性，并鼓励现场测试的媒介消除战略作为一个补充，正在进行的MDA的努力。淋巴丝虫病（LF）是一个全球性的健康问题，每年有超过1.2亿人受到影响。目前的LF消除计划的重点是通过年度大规模药物管理（MDA）向整个高危人群给予抗丝虫药物。虽然MDA计划在许多领域证明是有效的，但其他领域可能需要加强措施，如病媒控制。后者的一个例子是南太平洋的一些地区，那里波利尼西亚伊蚊是主要媒介。在这里，我们描述了一种新的载体控制方法的基础上自然发生的沃尔巴克氏体细菌感染。沃尔巴克氏体是一种内共生的细胞内细菌，可导致一种称为细胞质不相容性的不育形式。我们发现，与感染不同类型沃尔巴克氏体的蚊子进行基因渗入杂交，结果产生了A。在一些实施方案中，本发明涉及与自然感染的蚊子不相容的波利尼西亚蚊株（命名为“CP”）。在实验室测定中，CP雄性和野生型雄性之间的交配竞争力没有差异。结果支持继续发展该战略，作为改善公共卫生的工具。
Lymphatic filariasis (LF) is a leading cause of disability in South Pacific regions, where >96% of the 1.7 million population are at risk of LF infection. As part of current global campaign, mass drug administration (MDA) has effectively reduced lymphatic filiariasis prevalence, but mosquito vector biology can complicate the MDA strategy. In some regions, there is evidence that the goal of LF elimination cannot be attained via MDA alone. Obligate vector mosquitoes provide additional targets for breaking the LF transmission cycle, but existing methods are ineffective for controlling the primary vector throughout much of the South Pacific, Aedes polynesiensis. Here we demonstrate that interspecific hybridization and introgression results in an A. polynesiensis strain (‘CP’ strain) that is stably infected with the endosymbiotic Wolbachia bacteria from Aedes riversi. The CP strain is bi-directionally incompatible with naturally infected mosquitoes, resulting in female sterility. Laboratory assays demonstrate that CP males are equally competitive, resulting in population elimination when CP males are introduced into wild type A. polynesiensis populations. The findings demonstrate strategy feasibility and encourage field tests of the vector elimination strategy as a supplement to ongoing MDA efforts. Lymphatic filariasis (LF) is a global health problem, with over 120 million people affected annually. The current LF elimination program is focused on administering anti-filarial drugs to the entire at-risk population via annual mass drug administration (MDA). While the MDA program is proving effective in many areas, other areas may require augmentative measures such as vector control. An example of the latter is provided by some regions of the South Pacific where Aedes polynesiensis is the primary vector. Here, we describe a novel vector control approach based upon naturally occurring Wolbachia bacterial infections. Wolbachia are endosymbiotic intracellular bacteria that cause a form of sterility known as cytoplasmic incompatibility. We show that introgression crosses with mosquitoes that are infected with a different Wolbachia type results in an A. polynesiensis strain (designated ‘CP’) that is incompatible with naturally infected mosquitoes. No difference in mating competitiveness is observed between CP males and wild type males in laboratory assays. The results support continued development of the strategy as a tool to improve public health.
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影响因子：
56.9
作者：
Xi, ZY;Khoo, CCH;Dobson, SL
通讯作者：
Dobson, SL
影响因子：
--
作者：
Pichon, G
通讯作者：
Pichon, G
影响因子：
56.9
作者：
TRPIS, M;DUHRKOPF, RE;PARKER, KL
通讯作者：
PARKER, KL
DOI：
10.1016/s0035-9203(96)90106-6
发表时间：
1996-03-01
影响因子：
2.2
作者：
Vanamail, P;Ramaiah, KD;Bundy, DAP
通讯作者：
Bundy, DAP
影响因子：
3.3
作者：
Esterre, P;Plichart, C;Nguyen, NL
通讯作者：
Nguyen, NL