Duplication of a Pks gene cluster and subsequent functional diversification facilitate environmental adaptation in Metarhizium species.
Duplication of a Pks gene cluster and subsequent functional diversification facilitate environmental adaptation in Metarhizium species.
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Pks基因簇的复制和随后的功能多样化促进绿僵菌物种的环境适应
DOI：
10.1371/journal.pgen.1007472
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发表时间：
2018-06
期刊：
影响因子：
4.5
通讯作者：
Fang W
中科院分区：
文献类型：
--
作者：
Zeng G;Zhang P;Zhang Q;Zhao H;Li Z;Zhang X;Wang C;Yin WB;Fang W
真菌中合成次生代谢物的基因簇的复制和多样化的生态重要性仍然知之甚少。在这里，我们证明了基因簇的复制和随后的多样化在世界性真菌绿僵菌属中产生了两个聚酮合酶基因簇。多样化发生在两个 Pks 旁系同源物（Pks1 和 Pks2）的启动子区域和外显子-内含子结构中。这两个Pks基因具有不同的表达模式，Pks1在分生孢子期间高表达，Pks2在感染期间高表达。发现不同的上游信号通路调节这两个 Pks 基因。 Pks1 受 Hog1-MAPK、Slt2-MAPK 和 Mr-OPY2 正向调节，而 Pks2 受 Fus3-MAPK 正向调节，受 Mr-OPY2 负向调节。 Pks1和Pks2经过正选择并合成了不同的次级代谢产物。 PKS1 参与蒽醌衍生物的合成，并有助于分生孢子色素沉着，这在真菌对紫外线辐射和极端温度的耐受性中发挥着重要作用。 Pks2 基因的破坏延迟了感染结构的形成并增加了杀死昆虫所需的时间，表明 Pks2 有助于发病机制。因此，Pks基因簇的复制及其随后的功能多样化增加了绿僵菌物种的适应性灵活性。
The ecological importance of the duplication and diversification of gene clusters that synthesize secondary metabolites in fungi remains poorly understood. Here, we demonstrated that the duplication and subsequent diversification of a gene cluster produced two polyketide synthase gene clusters in the cosmopolitan fungal genus Metarhizium. Diversification occurred in the promoter regions and the exon-intron structures of the two Pks paralogs (Pks1 and Pks2). These two Pks genes have distinct expression patterns, with Pks1 highly expressed during conidiation and Pks2 highly expressed during infection. Different upstream signaling pathways were found to regulate the two Pks genes. Pks1 is positively regulated by Hog1-MAPK, Slt2-MAPK and Mr-OPY2, while Pks2 is positively regulated by Fus3-MAPK and negatively regulated by Mr-OPY2. Pks1 and Pks2 have been subjected to positive selection and synthesize different secondary metabolites. PKS1 is involved in synthesis of an anthraquinone derivative, and contributes to conidial pigmentation, which plays an important role in fungal tolerance to UV radiation and extreme temperatures. Disruption of the Pks2 gene delayed formation of infectious structures and increased the time taken to kill insects, indicating that Pks2 contributes to pathogenesis. Thus, the duplication of a Pks gene cluster and its subsequent functional diversification has increased the adaptive flexibility of Metarhizium species.
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影响因子：
4.5
作者：
Gao Q;Jin K;Ying SH;Zhang Y;Xiao G;Shang Y;Duan Z;Hu X;Xie XQ;Zhou G;Peng G;Luo Z;Huang W;Wang B;Fang W;Wang S;Zhong Y;Ma LJ;St Leger RJ;Zhao GP;Pei Y;Feng MG;Xia Y;Wang C
通讯作者：
Wang C
DOI：
10.1111/j.1478-4408.1998.tb01944.x
发表时间：
1998-02-01
期刊：
JOURNAL OF THE SOCIETY OF DYERS AND COLOURISTS
影响因子：
--
作者：
Hobson, DK;Wales, DS
通讯作者：
Wales, DS
影响因子：
3.4
作者：
Rangel, Drauzio E. N.;Butler, Michael J.;Roberts, Donald W.
通讯作者：
Roberts, Donald W.
影响因子：
3
作者：
Fang, Weiguo;Pava-Ripoll, Monica;Leger, Raymond St.
通讯作者：
Leger, Raymond St.
影响因子：
--
作者：
Fang, Weiguo;Bidochka, Michael J.
通讯作者：
Bidochka, Michael J.